BACKGROUND: Investigations were performed to assess the effect of poly-N-acetyl glucosamine (p-GlcNAc) fiber slurry-mediated hemostasis by interactions with red blood cells. METHODS: Red blood cell aggregation studies were performed using test material-coated microscope slides and multiphoton microscopic measurements. Enzymatic removal of red blood cell surface proteins was achieved using trypsin and neuraminidase treatment. Zeta-potential measurements (surface charge) were performed. RESULTS: Red blood cells interact directly with poly-N-acetyl glucosamine polymers through ionic interactions and cell-surface proteins. The effective concentration of poly-N-acetyl glucosamine fiber material for 50% red blood cell aggregation was 0.28 mg/mL. The p-GlcNAc beta-configuration fibers and an alpha-configuration structural modification of the fibers both produced maximal responses because of their zeta-potentials, whereas other chemically modified p-GlcNAcs and chitosans were ineffective. CONCLUSION: Poly-N-acetyl glucosamine-induced red blood cell aggregation is mediated by interactions with red blood cell surface charges.
BACKGROUND: Investigations were performed to assess the effect of poly-N-acetyl glucosamine (p-GlcNAc) fiber slurry-mediated hemostasis by interactions with red blood cells. METHODS:Red blood cell aggregation studies were performed using test material-coated microscope slides and multiphoton microscopic measurements. Enzymatic removal of red blood cell surface proteins was achieved using trypsin and neuraminidase treatment. Zeta-potential measurements (surface charge) were performed. RESULTS: Red blood cells interact directly with poly-N-acetyl glucosamine polymers through ionic interactions and cell-surface proteins. The effective concentration of poly-N-acetyl glucosamine fiber material for 50% red blood cell aggregation was 0.28 mg/mL. The p-GlcNAc beta-configuration fibers and an alpha-configuration structural modification of the fibers both produced maximal responses because of their zeta-potentials, whereas other chemically modified p-GlcNAcs and chitosans were ineffective. CONCLUSION:Poly-N-acetyl glucosamine-induced red blood cell aggregation is mediated by interactions with red blood cell surface charges.
Authors: Marina Burkatovskaya; George P Tegos; Emilia Swietlik; Tatiana N Demidova; Ana P Castano; Michael R Hamblin Journal: Biomaterials Date: 2006-04-17 Impact factor: 12.479
Authors: C A Nuss; J Huegel; J F Boorman-Padgett; D S Choi; S N Weiss; J Vournakis; L J Soslowsky Journal: Ann Biomed Eng Date: 2017-09-13 Impact factor: 3.934
Authors: Ferdinand I Broekema; Wim van Oeveren; Maaike H A Selten; Rolf J H Meijer; Joost T M de Wolf; Rudolf R M Bos Journal: Clin Oral Investig Date: 2012-08-02 Impact factor: 3.573
Authors: Ahad Sabab; Rajan Sundaresan Vediappan; John Finnie; C John McAdam; Alistair Jukes; Sarah Vreugde; Peter-John Wormald Journal: Front Surg Date: 2022-04-08