Literature DB >> 15280599

Production of antibodies against degradative neoepitopes in aggrecan.

John S Mort1, Peter J Roughley.   

Abstract

The use of synthetic peptides to generate rabbit polyclonal anticatabolic neoepitope antibodies that can be used to study the presence of defined proteolytic cleavage sites in aggrecan is described. Principles of peptide design and methods for preparation and characterization of ovalbumin conjugates are presented along with approaches for the characterization and affinity purification of the resulting antisera. Limitations associated with the use of antipeptide antibodies to study authentic protein neoepitopes are discussed.

Entities:  

Mesh：

Substances：

Year:  2004        PMID： 15280599     DOI： 10.1385/1-59259-810-2:237

Source DB:  PubMed          Journal:  Methods Mol Med        ISSN： 1543-1894


  4 in total

Review 1.  Lectican proteoglycans, their cleaving metalloproteinases, and plasticity in the central nervous system extracellular microenvironment.

Authors:  M D Howell; P E Gottschall
Journal:  Neuroscience       Date:  2012-05-22       Impact factor: 3.590

2.  MMPs are less efficient than ADAMTS5 in cleaving aggrecan core protein.

Authors:  Michaela Durigova; Hideaki Nagase; John S Mort; Peter J Roughley
Journal:  Matrix Biol       Date:  2010-11-03       Impact factor: 11.583

3.  Aggrecan, versican and type VI collagen are components of annular translamellar crossbridges in the intervertebral disc.

Authors:  James Melrose; Susan M Smith; Richard C Appleyard; Christopher B Little
Journal:  Eur Spine J       Date:  2007-10-31       Impact factor: 3.134

4.  Cleavage of type II collagen by cathepsin K in human osteoarthritic cartilage.

Authors:  Valeria M Dejica; John S Mort; Sheila Laverty; M David Percival; John Antoniou; David J Zukor; A Robin Poole
Journal:  Am J Pathol       Date:  2008-05-29       Impact factor: 4.307
  4 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.