A C Vidaeff1, S M Ramin, L C Gilstrap, J L Alcorn. 1. Division of Maternal-Fetal Medicine, Department of Obstetrics, Gynecology and Reproductive Sciences, University of Texas-Houston Medical School, Houston, Texas 77030, USA.
Abstract
OBJECTIVE: To determine whether the effect of a single 48-h exposure to dexamethasone in human lung cells is limited to 7-8 days. STUDY DESIGN: We used the NCI-H441 cell line, in which stability can be maintained beyond 7 days. The outcome was the stimulatory effect of dexamethasone on surfactant protein B (SP-B) gene transcription as expressed by SP-B mRNA accumulation. The experiment was conducted five times, in parallel with control. SP-B mRNA was determined at baseline, 48 h after dexamethasone exposure, and at 48-h intervals thereafter, up to 14 days, by quantitative reverse transcription polymerase chain reaction. Comparisons were made by the Mann-Whitney test. RESULTS: In conditions of our experiment, the inductive profile of SP-B mRNA after exposure to dexamethasone demonstrated maximal stimulation at 48 h (13-fold over control). Subsequently, there was a decline in mRNA, with return to near control levels by day 8, suggesting reversibility of dexamethasone action. CONCLUSION: Our data support the view that the surfactant-inducing properties of corticosteroids are limited to 7-8 days.
OBJECTIVE: To determine whether the effect of a single 48-h exposure to dexamethasone in human lung cells is limited to 7-8 days. STUDY DESIGN: We used the NCI-H441 cell line, in which stability can be maintained beyond 7 days. The outcome was the stimulatory effect of dexamethasone on surfactant protein B (SP-B) gene transcription as expressed by SP-B mRNA accumulation. The experiment was conducted five times, in parallel with control. SP-B mRNA was determined at baseline, 48 h after dexamethasone exposure, and at 48-h intervals thereafter, up to 14 days, by quantitative reverse transcription polymerase chain reaction. Comparisons were made by the Mann-Whitney test. RESULTS: In conditions of our experiment, the inductive profile of SP-B mRNA after exposure to dexamethasone demonstrated maximal stimulation at 48 h (13-fold over control). Subsequently, there was a decline in mRNA, with return to near control levels by day 8, suggesting reversibility of dexamethasone action. CONCLUSION: Our data support the view that the surfactant-inducing properties of corticosteroids are limited to 7-8 days.
Authors: Ashley N Battarbee; Stephanie T Ros; M Sean Esplin; Joseph Biggio; Radek Bukowski; Samuel Parry; Heping Zhang; Hao Huang; William Andrews; George Saade; Yoel Sadovsky; Uma M Reddy; Michael W Varner; Tracy A Manuck Journal: Am J Obstet Gynecol MFM Date: 2019-12-17
Authors: Andreas Ladenburger; Matthias Seehase; Boris W Kramer; Wolfgang Thomas; Johannes Wirbelauer; Christian P Speer; Steffen Kunzmann Journal: Am J Physiol Lung Cell Mol Physiol Date: 2010-08-06 Impact factor: 5.464