AIM: The aim of the study was to investigate the survival and growth of human breast carcinoma MCF-7 cells with different sensitivity to doxorubicin and production of transforming growth factor beta-(TGF-beta) in dependence on the dose- and duration of X-ray in order to check if the cross-resistance to doxorubicin and radiation effects exists. METHODS: Determination of cell number and valiability using trypan blue (0.1% (w/v)) exclusion method, Western blot analysis of p53 protein expression, biological testing of TGF-beta activity, lectinocytochemical analysis for apoptosis quantitative estimation in unirradiated and irradiated cells of both sublines of MCF-7 cells--sensitive (MCF-7(wt)) and resistant (MCF-7(DOX/R)) to doxorubicin. RESULTS: It was found that doxorubicin-resistant breast cancer cells were also more refractory to X-radiation-dependent growth inhibition. There were revealed different effects of distinct doses of X-ray on p53 protein expression by cells of both sublines. The level of production of TGF-beta was compared in non-irradiated MCF-7 cells and in these cells exposed to X-radiation. It was shown that X-radiation increased TGF-beta activity in the conditioned medium of the irradiated cells of both doxorubicin-sensitive and -resistant lines. CONCLUSIONS: The results of our study suggest that the biological effects of X-radiation on human breast cancer MCF-7 cells can be at least partly mediated by TGF-beta. Taking into account that TGF-beta is a potent natural immunosupressor, one may consider that an increased activity of this cytokine can intensify negative effects of X-radiation.
AIM: The aim of the study was to investigate the survival and growth of human breast carcinoma MCF-7 cells with different sensitivity to doxorubicin and production of transforming growth factor beta-(TGF-beta) in dependence on the dose- and duration of X-ray in order to check if the cross-resistance to doxorubicin and radiation effects exists. METHODS: Determination of cell number and valiability using trypan blue (0.1% (w/v)) exclusion method, Western blot analysis of p53 protein expression, biological testing of TGF-beta activity, lectinocytochemical analysis for apoptosis quantitative estimation in unirradiated and irradiated cells of both sublines of MCF-7 cells--sensitive (MCF-7(wt)) and resistant (MCF-7(DOX/R)) to doxorubicin. RESULTS: It was found that doxorubicin-resistant breast cancer cells were also more refractory to X-radiation-dependent growth inhibition. There were revealed different effects of distinct doses of X-ray on p53 protein expression by cells of both sublines. The level of production of TGF-beta was compared in non-irradiated MCF-7 cells and in these cells exposed to X-radiation. It was shown that X-radiation increased TGF-beta activity in the conditioned medium of the irradiated cells of both doxorubicin-sensitive and -resistant lines. CONCLUSIONS: The results of our study suggest that the biological effects of X-radiation on humanbreast cancer MCF-7 cells can be at least partly mediated by TGF-beta. Taking into account that TGF-beta is a potent natural immunosupressor, one may consider that an increased activity of this cytokine can intensify negative effects of X-radiation.
Authors: F Cabillic; F Bouet-Toussaint; O Toutirais; N Rioux-Leclercq; P Fergelot; C Thomas de la Pintière; N Genetet; J-J Patard; V Catros-Quemener Journal: Clin Exp Immunol Date: 2006-12 Impact factor: 4.330
Authors: Yuhua Wen; Zhan Chen; Jianfen Lu; Elizabeth Ables; Jean-Luc Scemama; Li V Yang; Jun Q Lu; Xin-Hua Hu Journal: PLoS One Date: 2017-09-08 Impact factor: 3.240