Responses of isolated human epigastric arteries to histamine.

Responses to histamine have been studied on ring preparations of epigastric artery obtained from normal and from pregnancy-induced hypertensive (PIH) women to characterize the mode of action of histamine in this vascular preparation. In non-contracted arterial rings, histamine elicited concentration-dependent H1 receptor-mediated contractions, competitively antagonised by mepyramine and cicletanine with pA2 values of 9.1 and 7.5, respectively. Arterial rings from pregnancy-induced hypertensive patients displayed greater sensitivity to histamine, but no change in maximal contractions, and were (at the EC30 histamine response) more susceptible to antagonism by mepyramine and cicletanine. Following precontraction by noradrenaline, histamine elicited relaxation responses only in the presence of H1 receptor antagonists. Endothelium removal or exposure to methylene blue significantly attenuated histamine-induced relaxation; the residual relaxations under these conditions appear to be due to a direct effect on H2 receptor on smooth muscle cells. The results show that, in human epigastric artery, histamine elicits H1 receptor-mediated contractions and that, following NA precontraction and in the presence of H1 receptor blockade, relaxations occur. Arterial rings from pregnancy-induced hypertensive patients showed modest but somewhat greater sensitivity to histamine, as well as being more susceptible to cicletanine and mepyramine.