Literature DB >> 15269534

Chitosan/gelatin composite microcarrier for hepatocyte culture.

Keguo Li1, Yun Wang, Zhenchuan Miao, Dayong Xu, Yuefeng Tang, Meifu Feng.   

Abstract

Solid and porous chitosan/gelatin (CG) composite microcarriers were prepared by a water-in-oil emulsion process with additional freezing and lyophilization. Adult rat hepatocytes (10(6) cells ml(-1)) attached on CG microcarriers maintained at least 15 d of viability and differentiated functions. Over 15 d, unimmobilized hepatocytes released 1.34-fold less lactate dehydrogenase (LDH), and retained 1.63-, 1.51- and 1.28-fold higher albumin secretion, urea synthesis and 7-ethoxycoumarin deethylation activities, respectively, than those on collagen-coated microcarriers. The CG matrix is therefore a promising microcarrier for hepatocyte culture.

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Year:  2004        PMID： 15269534     DOI： 10.1023/b:bile.0000025896.61490.6d

Source DB:  PubMed          Journal:  Biotechnol Lett        ISSN： 0141-5492            Impact factor:   2.461


  12 in total

1.  Biomimetic design and fabrication of porous chitosan–gelatin liver scaffolds with hierarchical channel network.

Authors:  Haibo Gong; Jephte Agustin; David Wootton; Jack G Zhou
Journal:  J Mater Sci Mater Med       Date:  2014-01       Impact factor: 3.896

2.  Long-term culture of primary hepatocytes: new matrices and microfluidic devices.

Authors:  Britta Burkhardt; Juan José Martinez-Sanchez; Anastasia Bachmann; Ruth Ladurner; Andreas K Nüssler
Journal:  Hepatol Int       Date:  2013-11-21       Impact factor: 6.047

3.  Biomaterials for liver tissue engineering.

Authors:  Era Jain; Apeksha Damania; Ashok Kumar
Journal:  Hepatol Int       Date:  2013-12-27       Impact factor: 6.047

4.  Effect of PEDOT:PSS in tissue engineering composite scaffold on improvement and maintenance of endothelial cell function.

Authors:  Masoumeh Hesam Mahmoudinezhad; Akbar Karkhaneh; Khosrow Jadidi
Journal:  J Biosci       Date:  2018-06       Impact factor: 1.826

5.  Expansion and Harvesting of hMSC-TERT.

Authors:  Christian Weber; Sebastian Pohl; Ralf Pörtner; Christine Wallrapp; Moustapha Kassem; Peter Geigle; Peter Czermak
Journal:  Open Biomed Eng J       Date:  2007-09-07

6.  Preparation and HL-7702 cell functionality of titania/chitosan composite scaffolds.

Authors:  Li Zhao; Jiang Chang; Wanyin Zhai
Journal:  J Mater Sci Mater Med       Date:  2008-11-26       Impact factor: 3.896

7.  Microfluidics generation of chitosan microgels containing glycerylphytate crosslinker for in situ human mesenchymal stem cells encapsulation.

Authors:  Ana Mora-Boza; Lina M Mancipe Castro; Rebecca S Schneider; Woojin M Han; Andrés J García; Blanca Vázquez-Lasa; Julio San Román
Journal:  Mater Sci Eng C Mater Biol Appl       Date:  2020-11-10       Impact factor: 7.328

8.  In vitro biodegradation and biocompatibility of gelatin/montmorillonite-chitosan intercalated nanocomposite.

Authors:  Hong Zhuang; Jun Ping Zheng; Hong Gao; Kang De Yao
Journal:  J Mater Sci Mater Med       Date:  2007-01-13       Impact factor: 4.727

9.  In Situ Transplantation of Alginate Bioencapsulated Adipose Tissues Derived Stem Cells (ADSCs) via Hepatic Injection in a Mouse Model.

Authors:  Mong-Jen Chen; Yuanqing Lu; Nicholas E Simpson; Mark J Beveridge; Ahmed S Elshikha; Mohammad Ahsanul Akbar; Hsin-Yin Tsai; Stephanie Hinske; Junling Qin; Christian R Grunwitz; Tina Chen; Mark L Brantly; Sihong Song
Journal:  PLoS One       Date:  2015-09-15       Impact factor: 3.240

Review 10.  3D Cultivation Techniques for Primary Human Hepatocytes.

Authors:  Anastasia Bachmann; Matthias Moll; Eric Gottwald; Cordula Nies; Roman Zantl; Helga Wagner; Britta Burkhardt; Juan J Martínez Sánchez; Ruth Ladurner; Wolfgang Thasler; Georg Damm; Andreas K Nussler
Journal:  Microarrays (Basel)       Date:  2015-02-16
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