Literature DB >> 15269525

Quantification of Saccharomyces cerevisiae viability using BacLight.

Tong Zhang1, Herbert H P Fang.   

Abstract

Yeast viability can be accurately quantified using BacLight, a kit which so far has been used only for bacterial analysis. Upon staining, viable cells can be differentiated from non-viable ones by either confocal laser scanning microscopy (CLSM), epifluorescence microscopy, or flow cytometry. Using Saccharomyces cerevisiae as a model, viabilities quantified by CLSM deviated an average of 1.7% from the actual data, and those determined by flow-cytometry by 1.4%.

Entities:  

Mesh:

Substances:

Year:  2004        PMID: 15269525     DOI: 10.1023/b:bile.0000030045.16713.19

Source DB:  PubMed          Journal:  Biotechnol Lett        ISSN: 0141-5492            Impact factor:   2.461


  8 in total

Review 1.  Flow cytometry applications in the food industry.

Authors:  Jaume Comas-Riu; Núria Rius
Journal:  J Ind Microbiol Biotechnol       Date:  2009-06-26       Impact factor: 3.346

Review 2.  Life, death, and in-between: meanings and methods in microbiology.

Authors:  Hazel M Davey
Journal:  Appl Environ Microbiol       Date:  2011-06-24       Impact factor: 4.792

3.  Fluidic Force Microscopy Demonstrates That Homophilic Adhesion by Candida albicans Als Proteins Is Mediated by Amyloid Bonds between Cells.

Authors:  Jérôme Dehullu; Claire Valotteau; Philippe Herman-Bausier; Melissa Garcia-Sherman; Maximilian Mittelviefhaus; Julia A Vorholt; Peter N Lipke; Yves F Dufrêne
Journal:  Nano Lett       Date:  2019-04-30       Impact factor: 11.189

4.  Assessment and interpretation of bacterial viability by using the LIVE/DEAD BacLight Kit in combination with flow cytometry.

Authors:  Michael Berney; Frederik Hammes; Franziska Bosshard; Hans-Ulrich Weilenmann; Thomas Egli
Journal:  Appl Environ Microbiol       Date:  2007-03-23       Impact factor: 4.792

5.  Novel image cytometric method for detection of physiological and metabolic changes in Saccharomyces cerevisiae.

Authors:  Leo L Chan; Alexandria Kury; Alisha Wilkinson; Charlotte Berkes; Alnoor Pirani
Journal:  J Ind Microbiol Biotechnol       Date:  2012-08-05       Impact factor: 3.346

6.  Time-resolved, single-cell analysis of induced and programmed cell death via non-invasive propidium iodide and counterstain perfusion.

Authors:  Christina E M Krämer; Wolfgang Wiechert; Dietrich Kohlheyer
Journal:  Sci Rep       Date:  2016-09-01       Impact factor: 4.379

7.  Selective Inhibition of Helicobacter pylori Carbonic Anhydrases by Carvacrol and Thymol Could Impair Biofilm Production and the Release of Outer Membrane Vesicles.

Authors:  Rossella Grande; Simone Carradori; Valentina Puca; Irene Vitale; Andrea Angeli; Alessio Nocentini; Alessandro Bonardi; Paola Gratteri; Paola Lanuti; Giuseppina Bologna; Pasquale Simeone; Clemente Capasso; Viviana De Luca; Claudiu T Supuran
Journal:  Int J Mol Sci       Date:  2021-10-27       Impact factor: 5.923

8.  Supercritical CO2 sterilization: An effective treatment to reprocess FFP3 face masks and to reduce waste during COVID-19 pandemic.

Authors:  Víctor Santos-Rosales; Clara López-Iglesias; Ana Sampedro-Viana; Carmen Alvarez-Lorenzo; Samaneh Ghazanfari; Beatriz Magariños; Carlos A García-González
Journal:  Sci Total Environ       Date:  2022-02-23       Impact factor: 10.753

  8 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.