Literature DB >> 15265749

An improvement of the site-directed mutagenesis method by combination of megaprimer, one-side PCR and DpnI treatment.

Dongsheng Wei1, Mingchun Li, Xinxin Zhang, Laijun Xing.   

Abstract

Mesh:

Substances:

Year:  2004        PMID: 15265749     DOI: 10.1016/j.ab.2004.04.019

Source DB:  PubMed          Journal:  Anal Biochem        ISSN: 0003-2697            Impact factor:   3.365


× No keyword cloud information.
  14 in total

1.  Efficient site-directed saturation mutagenesis using degenerate oligonucleotides.

Authors:  David L Steffens; John G K Williams
Journal:  J Biomol Tech       Date:  2007-07

2.  An easy-to-use site-directed mutagenesis method with a designed restriction site for convenient and reliable mutant screening.

Authors:  Bao-zhong Zhang; Xin Zhang; Xiao-ping An; Duo-liang Ran; Yu-sen Zhou; Jun Lu; Yi-gang Tong
Journal:  J Zhejiang Univ Sci B       Date:  2009-06       Impact factor: 3.066

3.  Long distance multiple-site directed plasmid mutagenesis by one-step PCR using non-overlapped primers.

Authors:  Yuling Bu; Hui Wang; Jing Li; Zhaoxin Zhang; Yinghe Hu; Zhiliang Xu
Journal:  Mol Biotechnol       Date:  2013-09       Impact factor: 2.695

4.  Modulation of translation initiation efficiency in classical swine fever virus.

Authors:  Martin Barfred Friis; Thomas Bruun Rasmussen; Graham J Belsham
Journal:  J Virol       Date:  2012-06-06       Impact factor: 5.103

5.  Crystal structure of the interferon gamma receptor alpha chain from chicken reveals an undetected extra helix compared with the human counterparts.

Authors:  Zhiguang Ping; Jianxun Qi; Yanling Sun; Guangwen Lu; Yi Shi; Xiaojia Wang; George F Gao; Ming Wang
Journal:  J Interferon Cytokine Res       Date:  2013-11-27       Impact factor: 2.607

6.  Thermostable DNA ligase-mediated PCR production of circular plasmid (PPCP) and its application in directed evolution via in situ error-prone PCR.

Authors:  Yilin Le; Huayou Chen; Robert Zagursky; J H David Wu; Weilan Shao
Journal:  DNA Res       Date:  2013-04-30       Impact factor: 4.458

7.  REPLACR-mutagenesis, a one-step method for site-directed mutagenesis by recombineering.

Authors:  Ashutosh Trehan; Michał Kiełbus; Jakub Czapinski; Andrzej Stepulak; Ilpo Huhtaniemi; Adolfo Rivero-Müller
Journal:  Sci Rep       Date:  2016-01-11       Impact factor: 4.379

8.  Role of the ArcAB two-component system in the resistance of Escherichia coli to reactive oxygen stress.

Authors:  Cindy Loui; Alexander C Chang; Sangwei Lu
Journal:  BMC Microbiol       Date:  2009-08-28       Impact factor: 3.605

9.  SpeedyGenes: an improved gene synthesis method for the efficient production of error-corrected, synthetic protein libraries for directed evolution.

Authors:  Andrew Currin; Neil Swainston; Philip J Day; Douglas B Kell
Journal:  Protein Eng Des Sel       Date:  2014-08-09       Impact factor: 1.650

Review 10.  Synthetic biology for the directed evolution of protein biocatalysts: navigating sequence space intelligently.

Authors:  Andrew Currin; Neil Swainston; Philip J Day; Douglas B Kell
Journal:  Chem Soc Rev       Date:  2015-03-07       Impact factor: 54.564

View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.