Development of perforated microthin poly(epsilon-caprolactone) films as matrices for membrane tissue engineering.

The design and fabrication of thin films based on bioresorbable polymers such as poly(epsilon-caprolactone) (PCL) has been the focus of a part of current biomedical research, especially as matrices for membrane tissue engineering. We have successfully developed perforated microthin PCL membrane for this purpose. Two critical issues are the control of moisture permeability and understanding the degradation of PCL microthin film. In order to increase the moisture permeability. PCL films were biaxially stretched to a thickness of 10 +/- 3 microm and perforated with uniform array of holes (180-275 microm) using a Sony Robotic system. After perforation, the water vapour transmission rate was increased by 50% to a value of 47.6 +/- 2.7 g/h per m2. Accelerated hydrolytic degradations were performed in 5 M NaOH. The degraded samples were characterised for changes in weight, surface morphology, mechanical properties, crystallinity and molecular weight. Hydrolytic degradation commenced with random chain scission of backbone ester bonds on the film surface and followed by loss of material due to surface erosion. In general, the perforated films degraded faster than the unperforated microthin films. Scanning electron microscopic images showed that surface erosion led to extensive formation of micropores, microcracks and increased in surface roughness.