Ming Wu1, Shuho Semba, Dong Li, Hiroshi Yokozaki. 1. Division of Surgical Pathology, Department of Biomedical Informatics, Kobe University Graduate School of Medicine, Kobe, Japan.
Abstract
OBJECTIVE: Mucinous adenocarcinomas (MACs) of the stomach usually show an invasive expansive growth and a poor prognosis. We examined the possibility of molecular pathological subtyping of MACs of the stomach. METHODS: Forty-one formalin-fixed and paraffin-embedded MAC specimens of the stomach were analyzed. Mucin subtypes (MUC2, CD10, HGM, M-GGMC-1) and expression levels of hMLH1, p53 and Ki-67 were analyzed by immunohistochemistry as well as genetic alterations in the p53 gene and microsatellite instability (MSI). RESULTS: According to both MSI and p53 status, these tumors were subclassified into three groups: the mutator-type tumors, the suppressor/p53-type tumors and the unclassified tumors. The mutator-type tumors demonstrated lower p53 expression and had lower proliferative activity than the suppressor/p53-type tumors, whereas most of the suppressor/p53-type tumors expressed CD10. However, there was no significant difference between the mutator- and suppressor/p53-type tumors in clinicopathological parameters including the patients' outcome. CONCLUSION: Our results indicate that MACs of the stomach are composed of at least three subtypes according to the molecular pathological background for their carcinogenesis. Further study of carcinomas with detailed morphological and biological phenotyping of each subtype may provide useful information for better clinical management. Copyright 2004 S. Karger AG, Basel
OBJECTIVE:Mucinous adenocarcinomas (MACs) of the stomach usually show an invasive expansive growth and a poor prognosis. We examined the possibility of molecular pathological subtyping of MACs of the stomach. METHODS: Forty-one formalin-fixed and paraffin-embedded MAC specimens of the stomach were analyzed. Mucin subtypes (MUC2, CD10, HGM, M-GGMC-1) and expression levels of hMLH1, p53 and Ki-67 were analyzed by immunohistochemistry as well as genetic alterations in the p53 gene and microsatellite instability (MSI). RESULTS: According to both MSI and p53 status, these tumors were subclassified into three groups: the mutator-type tumors, the suppressor/p53-type tumors and the unclassified tumors. The mutator-type tumors demonstrated lower p53 expression and had lower proliferative activity than the suppressor/p53-type tumors, whereas most of the suppressor/p53-type tumors expressed CD10. However, there was no significant difference between the mutator- and suppressor/p53-type tumors in clinicopathological parameters including the patients' outcome. CONCLUSION: Our results indicate that MACs of the stomach are composed of at least three subtypes according to the molecular pathological background for their carcinogenesis. Further study of carcinomas with detailed morphological and biological phenotyping of each subtype may provide useful information for better clinical management. Copyright 2004 S. Karger AG, Basel
Authors: Niek Hugen; Michiel Simons; Altuna Halilović; Rachel S van der Post; Anna J Bogers; Monica Aj Marijnissen-van Zanten; Johannes Hw de Wilt; Iris D Nagtegaal Journal: J Pathol Clin Res Date: 2014-11-05