OBJECTIVE: The expressions of minichromosome maintenance protein 2 (MCM2), Ki-67, and p53 were examined to analyze their pathobiological significance in human lung adenocarcinomas. METHODS: We performed Western blot analysis in six human lung adenocarcinoma cell lines and immunohistochemistry in 145 surgically removed adenocarcinomas to examine the MCM2 expression. Labeling indices (LIs; %) of MCM2, Ki-67, and p53 in the tumor cells were compared with clinicopathological profiles and overall survival rates. RESULTS: MCM2 protein was detected in all cell lines examined, with specific bands. MCM2 LIs were significantly correlated with sex, histological type, differentiation, pathological stage, and LIs of Ki-67 and p53 (p < 0.05). Significantly higher LIs of MCM2 and Ki-67 were noted in the 122 non-pure bronchioloalveolar carcinomas than in the 23 pure bronchioloalveolar carcinomas (p < 0.01), and the prognosis was poorer in the former than in the latter (p < 0.01). Sex, pathological stage, and high LIs of MCM2 and/or Ki-67 were independent prognostic factors (p < 0.05). CONCLUSION: High LIs of MCM2 and/or Ki-67 suggest a poor prognosis in patients with lung adenocarcinoma (non-pure bronchioloalveolar carcinoma). Copyright 2004 S. Karger AG, Basel
OBJECTIVE: The expressions of minichromosome maintenance protein 2 (MCM2), Ki-67, and p53 were examined to analyze their pathobiological significance in humanlung adenocarcinomas. METHODS: We performed Western blot analysis in six humanlung adenocarcinoma cell lines and immunohistochemistry in 145 surgically removed adenocarcinomas to examine the MCM2 expression. Labeling indices (LIs; %) of MCM2, Ki-67, and p53 in the tumor cells were compared with clinicopathological profiles and overall survival rates. RESULTS:MCM2 protein was detected in all cell lines examined, with specific bands. MCM2 LIs were significantly correlated with sex, histological type, differentiation, pathological stage, and LIs of Ki-67 and p53 (p < 0.05). Significantly higher LIs of MCM2 and Ki-67 were noted in the 122 non-pure bronchioloalveolar carcinomas than in the 23 pure bronchioloalveolar carcinomas (p < 0.01), and the prognosis was poorer in the former than in the latter (p < 0.01). Sex, pathological stage, and high LIs of MCM2 and/or Ki-67 were independent prognostic factors (p < 0.05). CONCLUSION: High LIs of MCM2 and/or Ki-67 suggest a poor prognosis in patients with lung adenocarcinoma (non-pure bronchioloalveolar carcinoma). Copyright 2004 S. Karger AG, Basel
Authors: Walter N Hittelman; Diane D Liu; Jonathan M Kurie; Reuben Lotan; Jin Soo Lee; Fadlo Khuri; Heladio Ibarguen; Rodolfo C Morice; Garrett Walsh; Jack A Roth; John Minna; Jae Y Ro; Anita Broxson; Waun Ki Hong; J Jack Lee Journal: J Natl Cancer Inst Date: 2007-10-30 Impact factor: 13.506
Authors: Ellen C Obermann; Philip Went; Annette Zimpfer; Alexandar Tzankov; Peter J Wild; Robert Stoehr; Stefano A Pileri; Stephan Dirnhofer Journal: BMC Cancer Date: 2005-12-20 Impact factor: 4.430
Authors: Jun Yang; Nithya Ramnath; Kirsten B Moysich; Harold L Asch; Helen Swede; Sadir J Alrawi; Joel Huberman; Joseph Geradts; John S J Brooks; Dongfeng Tan Journal: BMC Cancer Date: 2006-08-01 Impact factor: 4.430