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Literature DB >> 15256599

A large conformational change of the translocation ATPase SecA.

Andrew R Osborne¹, William M Clemons, Tom A Rapoport.

Abstract

The ATPase SecA mediates the posttranslational translocation of a wide range of polypeptide substrates through the SecY channel in the cytoplasmic membrane of bacteria. We have determined the crystal structure of a monomeric form of Bacillus subtilis SecA at a 2.2-A resolution. A comparison with the previously determined structures of SecA reveals a nucleotide-independent, large conformational change that opens a deep groove similar to that in other proteins that interact with diverse polypeptides. We propose that the open form of SecA represents an activated state.

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Year: 2004 PMID： 15256599 PMCID： PMC491988 DOI： 10.1073/pnas.0401742101

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

40 in total

1. A molecular switch in SecA protein couples ATP hydrolysis to protein translocation.

Authors: S Karamanou; E Vrontou; G Sianidis; C Baud; T Roos; A Kuhn; A S Politou; A Economou
Journal: Mol Microbiol Date: 1999-12 Impact factor: 3.501

2. High-resolution solution structure of the 18 kDa substrate-binding domain of the mammalian chaperone protein Hsc70.

Authors: R C Morshauser; W Hu; H Wang; Y Pang; G C Flynn; E R Zuiderweg
Journal: J Mol Biol Date: 1999-06-25 Impact factor: 5.469

3. Cross-talk between catalytic and regulatory elements in a DEAD motor domain is essential for SecA function.

Authors: G Sianidis; S Karamanou; E Vrontou; K Boulias; K Repanas; N Kyrpides; A S Politou; A Economou
Journal: EMBO J Date: 2001-03-01 Impact factor: 11.598

4. Crystal structures of complexes of PcrA DNA helicase with a DNA substrate indicate an inchworm mechanism.

Authors: S S Velankar; P Soultanas; M S Dillingham; H S Subramanya; D B Wigley
Journal: Cell Date: 1999-04-02 Impact factor: 41.582

5. Escherichia coli SecA helicase activity is not required in vivo for efficient protein translocation or autogenous regulation.

Authors: M O Schmidt; R M Brosh; D B Oliver
Journal: J Biol Chem Date: 2001-07-27 Impact factor: 5.157

6. Genetic dissection of SecA: suppressor mutations against the secY205 translocase defect.

Authors: G Matsumoto; H Nakatogawa; H Mori; K Ito
Journal: Genes Cells Date: 2000-12 Impact factor: 1.891

7. Tyr-326 plays a critical role in controlling SecA-preprotein interaction.

Authors: L Kourtz; D Oliver
Journal: Mol Microbiol Date: 2000-09 Impact factor: 3.501

8. Lipid and signal peptide-induced conformational changes within the C-domain of Escherichia coli SecA protein.

Authors: H Ding; I Mukerji; D Oliver
Journal: Biochemistry Date: 2001-02-13 Impact factor: 3.162

9. The high affinity ATP binding site modulates the SecA-precursor interaction.

Authors: F van Voorst; I J Vereyken; B de Kruijff
Journal: FEBS Lett Date: 2000-12-01 Impact factor: 4.124

10. Crystallographic and calorimetric analysis of peptide binding to OppA protein.

Authors: S H Sleigh; P R Seavers; A J Wilkinson; J E Ladbury; J R Tame
Journal: J Mol Biol Date: 1999-08-13 Impact factor: 5.469

64 in total

Review 1. The bacterial Sec-translocase: structure and mechanism.

Authors: Jelger A Lycklama A Nijeholt; Arnold J M Driessen
Journal: Philos Trans R Soc Lond B Biol Sci Date: 2012-04-19 Impact factor: 6.237

2. Direct identification of the site of binding on the chaperone SecB for the amino terminus of the translocon motor SecA.

Authors: Linda L Randall; Michael T Henzl
Journal: Protein Sci Date: 2010-06 Impact factor: 6.725

3. Orientation of SecA and SecB in complex, derived from disulfide cross-linking.

Authors: Yuying Suo; Simon J S Hardy; Linda L Randall
Journal: J Bacteriol Date: 2010-10-29 Impact factor: 3.490

4. Using a low denaturant model to explore the conformational features of translocation-active SecA.

Authors: Jenny L Maki; Beena Krishnan; Lila M Gierasch
Journal: Biochemistry Date: 2012-02-08 Impact factor: 3.162

5. Structural Similarities and Differences between Two Functionally Distinct SecA Proteins, Mycobacterium tuberculosis SecA1 and SecA2.

Authors: Stephanie Swanson; Thomas R Ioerger; Nathan W Rigel; Brittany K Miller; Miriam Braunstein; James C Sacchettini
Journal: J Bacteriol Date: 2015-12-14 Impact factor: 3.490