OBJECTIVE: To study genetic damage of mice caused by the volatile organic compounds (VOC) of decoration materials. METHODS: Fifty-five hotel guest rooms newly decorated within 6 months and 18 hotel guest rooms not decorated within 3 years were selected to determine the concentrations of 6 main VOC (benzene, methylbenzene, dimethylbenzene, ethyl acetate, butyl acetate, formaldehyde) in the air. Mice were exposed to VOC with the concentrations of 5, 10, 20, 40 times respectively as high as those present in the newly decorated rooms in an exposure cabinet for 15 days. DNA damage of peripheral lymphocytes of the mice was determined by single cell gel electrophoresis (SCGE) and bone marrow micronucleus test. RESULTS: The concentrations of benzene, methylbenzene, dimethylbenzene, ethyl acetate, butyl acetate and formaldehyde in the rooms newly decorated within 6 months (6.50, 3.00, 6.70, 41.33, 1.70 and 0.14 mg/m(3) respectively) were significantly higher than those in rooms not decorated within 3 years (0.08, 0.94, 1.38, 0.25, 0.25, 0.01 mg/m(3), P < 0.01). DNA damage rates of peripheral lymphocytes in the concentrations of 10, 20, 40 times of exposure groups were significantly higher than those in the control groups (P < 0.05 or P < 0.01), and the frequencies of micronucleus in the mice exposed to 40 times of concentration was significantly higher than that in control group. CONCLUSION: High concentrations of the volatile organic chemical compounds may cause genetic damage in mice. SCGE test is more sensitive than micronucleus test.
OBJECTIVE: To study genetic damage of mice caused by the volatile organic compounds (VOC) of decoration materials. METHODS: Fifty-five hotel guest rooms newly decorated within 6 months and 18 hotel guest rooms not decorated within 3 years were selected to determine the concentrations of 6 main VOC (benzene, methylbenzene, dimethylbenzene, ethyl acetate, butyl acetate, formaldehyde) in the air. Mice were exposed to VOC with the concentrations of 5, 10, 20, 40 times respectively as high as those present in the newly decorated rooms in an exposure cabinet for 15 days. DNA damage of peripheral lymphocytes of the mice was determined by single cell gel electrophoresis (SCGE) and bone marrow micronucleus test. RESULTS: The concentrations of benzene, methylbenzene, dimethylbenzene, ethyl acetate, butyl acetate and formaldehyde in the rooms newly decorated within 6 months (6.50, 3.00, 6.70, 41.33, 1.70 and 0.14 mg/m(3) respectively) were significantly higher than those in rooms not decorated within 3 years (0.08, 0.94, 1.38, 0.25, 0.25, 0.01 mg/m(3), P < 0.01). DNA damage rates of peripheral lymphocytes in the concentrations of 10, 20, 40 times of exposure groups were significantly higher than those in the control groups (P < 0.05 or P < 0.01), and the frequencies of micronucleus in the mice exposed to 40 times of concentration was significantly higher than that in control group. CONCLUSION: High concentrations of the volatile organic chemical compounds may cause genetic damage in mice. SCGE test is more sensitive than micronucleus test.
Authors: Mahmoud A Mahmoud; Abeer H Abd El-Rahim; Karima F Mahrous; Mohamed Abdelsalam; Nashwa A Abu-Aita; Mamdouh Afify Journal: Environ Sci Pollut Res Int Date: 2019-05-10 Impact factor: 4.223
Authors: Luoping Zhang; Xiaojiang Tang; Nathaniel Rothman; Roel Vermeulen; Zhiying Ji; Min Shen; Chuangyi Qiu; Weihong Guo; Songwang Liu; Boris Reiss; Laura Beane Freeman; Yichen Ge; Alan E Hubbard; Ming Hua; Aaron Blair; Noe Galvan; Xiaolin Ruan; Blanche P Alter; Kerry X Xin; Senhua Li; Lee E Moore; Sungkyoon Kim; Yuxuan Xie; Richard B Hayes; Mariko Azuma; Michael Hauptmann; Jun Xiong; Patricia Stewart; Laiyu Li; Stephen M Rappaport; Hanlin Huang; Joseph F Fraumeni; Martyn T Smith; Qing Lan Journal: Cancer Epidemiol Biomarkers Prev Date: 2010-01 Impact factor: 4.254