BACKGROUND: The hypoosmotic swelling test (HOST) was recommended as a predictive test for in vitro fertilization (IVF) outcome. These results, however, were controversial and the results for thawed semen were insufficient. The present study was conducted in order to clarify the predictive value of the HOST for IVF in fresh and thawed sperm. METHODS: The hypoosmotic swelling test was performed in three groups: husband's fresh semen "subfertile" group, donor's thawed semen group, and donor's fresh semen "fertile" group. RESULTS: No correlation was found between HOST values and sperm characteristics in fresh or thawed sperm. Fresh sperm HOST values correlated with IVF fertilizations. No such correlations were found when thawed sperm was used. HOST values were significantly higher in the fresh fertile donor group than in the fresh subfertile group (P less than 0.001). Following the freezing and thawing process, HOST values decreased dramatically. Nevertheless, the fertilization rate was still higher compared to that of the fresh subfertile group (P less than 0.001). There were significantly more IVF cycles with no fertilizations when HOST values were below 45%. CONCLUSION: The HOST has a predictive value for fertilization of oocytes in IVF cycles when fresh semen, but not thawed sperm, is used. The freezing-thawing process affects the outer membrane of the spermatozoon and changes its characteristics, leading to a decrease in HOST values. Sperm characteristics that play a role in the fertilization process are not expressed directly by HOST values.
BACKGROUND: The hypoosmotic swelling test (HOST) was recommended as a predictive test for in vitro fertilization (IVF) outcome. These results, however, were controversial and the results for thawed semen were insufficient. The present study was conducted in order to clarify the predictive value of the HOST for IVF in fresh and thawed sperm. METHODS: The hypoosmotic swelling test was performed in three groups: husband's fresh semen "subfertile" group, donor's thawed semen group, and donor's fresh semen "fertile" group. RESULTS: No correlation was found between HOST values and sperm characteristics in fresh or thawed sperm. Fresh sperm HOST values correlated with IVF fertilizations. No such correlations were found when thawed sperm was used. HOST values were significantly higher in the fresh fertile donor group than in the fresh subfertile group (P less than 0.001). Following the freezing and thawing process, HOST values decreased dramatically. Nevertheless, the fertilization rate was still higher compared to that of the fresh subfertile group (P less than 0.001). There were significantly more IVF cycles with no fertilizations when HOST values were below 45%. CONCLUSION: The HOST has a predictive value for fertilization of oocytes in IVF cycles when fresh semen, but not thawed sperm, is used. The freezing-thawing process affects the outer membrane of the spermatozoon and changes its characteristics, leading to a decrease in HOST values. Sperm characteristics that play a role in the fertilization process are not expressed directly by HOST values.
Authors: I Hirsch; W E Gibbons; L I Lipshultz; K K Rossavik; R L Young; A N Poindexter; M G Dodson; W E Findley Journal: Fertil Steril Date: 1986-05 Impact factor: 7.329
Authors: Rafael Favero Ambar; Marcello M Gava; Milton Ghirelli-Filho; Ivan H Yoshida; Thais Serzedello De Paula; Sidney Glina Journal: Arab J Urol Date: 2021-07-22
Authors: Ashok Agarwal; Rakesh K Sharma; Sajal Gupta; Florence Boitrelle; Renata Finelli; Neel Parekh; Damayanthi Durairajanayagam; Ramadan Saleh; Mohamed Arafa; Chak Lam Cho; Ala'a Farkouh; Amarnath Rambhatla; Ralf Henkel; Paraskevi Vogiatzi; Nicholas Tadros; Parviz Kavoussi; Edmund Ko; Kristian Leisegang; Hussein Kandil; Ayad Palani; Gianmaria Salvio; Taymour Mostafa; Osvaldo Rajmil; Saleem Ali Banihani; Samantha Schon; Tan V Le; Ponco Birowo; Gökhan Çeker; Juan Alvarez; Juan Manuel Corral Molina; Christopher C K Ho; Aldo E Calogero; Kareim Khalafalla; Mesut Berkan Duran; Shinnosuke Kuroda; Giovanni M Colpi; Armand Zini; Christina Anagnostopoulou; Edoardo Pescatori; Eric Chung; Ettore Caroppo; Fotios Dimitriadis; Germar-Michael Pinggera; Gian Maria Busetto; Giancarlo Balercia; Haitham Elbardisi; Hisanori Taniguchi; Hyun Jun Park; Israel Maldonado Rosas; Jean de la Rosette; Jonathan Ramsay; Kasonde Bowa; Mara Simopoulou; Marcelo Gabriel Rodriguez; Marjan Sabbaghian; Marlon Martinez; Mohamed Ali Sadighi Gilani; Mohamed S Al-Marhoon; Raghavender Kosgi; Rossella Cannarella; Sava Micic; Shinichiro Fukuhara; Sijo Parekattil; Sunil Jindal; Taha Abo-Almagd Abdel-Meguid; Yoshiharu Morimoto; Rupin Shah Journal: World J Mens Health Date: 2021-10-13 Impact factor: 5.400