Literature DB >> 15253433

Proteomic analysis of Trypanosoma cruzi developmental stages using isotope-coded affinity tag reagents.

Jaime Paba1, Carlos A O Ricart, Wagner Fontes, Jaime M Santana, Antonio R L Teixeira, Jason Marchese, Brian Williamson, Tony Hunt, Barry L Karger, Marcelo V Sousa.   

Abstract

Comparative proteome analysis of developmental stages of the human pathogen Trypanosoma cruzi was carried out by isotope-coded affinity tag technology (ICAT) associated with liquid cromatography-mass spectrometry peptide sequencing (LC-MS/MS). Protein extracts of the protozoan trypomastigote and amastigote stages were labeled with heavy (D8) and light (D0) ICAT reagents and subjected to cation exchange and avidin affinity chromatographies followed by LC-MS/MS analysis. High confidence sequence information and expression levels for 41 T. cruzi polypeptides, including metabolic enzymes, paraflagellar rod components, tubulins, and heat-shock proteins were reported. Twenty-nine proteins displayed similar levels of expression in both forms of the parasite, nine proteins presented higher levels in trypomastigotes, whereas three were more expressed in amastigotes.

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Year:  2004        PMID： 15253433     DOI： 10.1021/pr034075o

Source DB:  PubMed          Journal:  J Proteome Res        ISSN： 1535-3893            Impact factor:   4.466


  10 in total

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Journal:  J Bacteriol       Date:  2007-02-02       Impact factor: 3.490

Review 2.  Pathogenesis of chagas' disease: parasite persistence and autoimmunity.

Authors:  Antonio R L Teixeira; Mariana M Hecht; Maria C Guimaro; Alessandro O Sousa; Nadjar Nitz
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3.  The steady-state transcriptome of the four major life-cycle stages of Trypanosoma cruzi.

Authors:  Todd A Minning; D Brent Weatherly; James Atwood; Ron Orlando; Rick L Tarleton
Journal:  BMC Genomics       Date:  2009-08-07       Impact factor: 3.969

4.  Proteomic analysis of detergent-solubilized membrane proteins from insect-developmental forms of Trypanosoma cruzi.

Authors:  Esteban M Cordero; Ernesto S Nakayasu; Luciana G Gentil; Nobuko Yoshida; Igor C Almeida; José Franco da Silveira
Journal:  J Proteome Res       Date:  2009-07       Impact factor: 4.466

5.  Proteomics in Trypanosoma cruzi--localization of novel proteins to various organelles.

Authors:  Marcela Ferella; Daniel Nilsson; Hamid Darban; Claudia Rodrigues; Esteban J Bontempi; Roberto Docampo; Björn Andersson
Journal:  Proteomics       Date:  2008-07       Impact factor: 3.984

6.  Adhesion of Trypanosoma cruzi trypomastigotes to fibronectin or laminin modifies tubulin and paraflagellar rod protein phosphorylation.

Authors:  Eliciane C Mattos; Robert I Schumacher; Walter Colli; Maria Julia M Alves
Journal:  PLoS One       Date:  2012-10-04       Impact factor: 3.240

7.  Proteomic analysis of the ventral disc of Giardia lamblia.

Authors:  Daniela Lourenço; Iamara da Silva Andrade; Letícia Labati Terra; Patricia Ramos Guimarães; Russolina Benedeta Zingali; Wanderley de Souza
Journal:  BMC Res Notes       Date:  2012-01-19

8.  Trypanosoma cruzi alkaline 2-DE: Optimization and application to comparative proteome analysis of flagellate life stages.

Authors:  Adriana D Magalhães; Sébastien Charneau; Jaime Paba; Rafael A P Guércio; Antonio R L Teixeira; Jaime M Santana; Marcelo V Sousa; Carlos A O Ricart
Journal:  Proteome Sci       Date:  2008-09-08       Impact factor: 2.480

9.  Analysis of Phaseolus vulgaris response to its association with Trichoderma harzianum (ALL-42) in the presence or absence of the phytopathogenic fungi Rhizoctonia solani and Fusarium solani.

Authors:  Jackeline L Pereira; Rayner M L Queiroz; Sébastien O Charneau; Carlos R Felix; Carlos A O Ricart; Francilene Lopes da Silva; Andrei Stecca Steindorff; Cirano J Ulhoa; Eliane F Noronha
Journal:  PLoS One       Date:  2014-05-30       Impact factor: 3.240

10.  Proteome analysis of Phytomonas serpens, a phytoparasite of medical interest.

Authors:  Agenor de Castro Moreira Dos Santos Júnior; Carlos André Ornelas Ricart; Arthur Henriques Pontes; Wagner Fontes; Agnelo Rodrigues de Souza; Mariana Souza Castro; Marcelo Valle de Sousa; Beatriz Dolabela de Lima
Journal:  PLoS One       Date:  2018-10-10       Impact factor: 3.240
  10 in total

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