| Literature DB >> 15250702 |
Marian C Bryan1, Fabio Fazio, Hing-Ken Lee, Cheng-Yuan Huang, Aileen Chang, Michael D Best, Daniel A Calarese, Ola Blixt, James C Paulson, Dennis Burton, Ian A Wilson, Chi-Huey Wong.
Abstract
A covalent array for the display of complex oligosaccharides in microtiter plates has been developed. This strategy is conducive to the display of carbohydrates to proteins of interest such as lectins and antibodies, including the broadly neutralizing antibody 2G12 against HIV envelope oligomannose and can be cleaved from the surface for further characterization by mass spectrometry. The system was used to probe the multivalent interaction of 2G12 with an optimal epitope (Kd 0.1 muM).Entities:
Mesh:
Substances:
Year: 2004 PMID: 15250702 DOI: 10.1021/ja048433f
Source DB: PubMed Journal: J Am Chem Soc ISSN: 0002-7863 Impact factor: 15.419