| Literature DB >> 15249067 |
Luiza M Araújo1, Rose A Monteiro, Emanuel M Souza, M Berenice R Steffens, Liu U Rigo, Fábio O Pedrosa, Leda S Chubatsu.
Abstract
The Azospirillum brasilense transcription regulator NifA and the nitrogen-status signaling proteins GlnB, GlnZ and GlnK were expressed in Escherichia coli and analyzed for their ability to activate nif gene expression. When expressed separately, none of the proteins were able to activate nifH promoter expression in any tested conditions; in contrast, nifH expression was observed in cells grown in the absence of ammonium and oxygen and when expressing simultaneously NifA and GlnB proteins, but not when expressing NifA and GlnZ or GlnK. Our results show that the GlnB protein is required for transcription activation by Azospirillum brasilense NifA and it cannot be replaced by GlnZ or GlnK.Entities:
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Year: 2004 PMID： 15249067 DOI： 10.1016/j.resmic.2004.03.002
Source DB: PubMed Journal: Res Microbiol ISSN： 0923-2508 Impact factor: 3.992