OBJECTIVE: T-box expressed in T cells (T-bet) is a transcription factor regulating the commitment of T helper (Th) cells by driving the cells into the Th1 direction. Abnormal Th1/Th2 balance may lead to complex disorders like asthma or autoimmune diseases. Recent studies have suggested that T-bet might be a candidate gene for asthma. This led us to screen 23 Finnish individuals for single-nucleotide polymorphisms (SNPs) in the T-bet locus and study the association between the SNPs and high serum IgE level and asthma. METHODS: We screened all six exons, adjacent intronic areas and 2 kb of the 5'-flanking region from 23 individuals utilizing WAVE trade mark technology. To explore whether T-bet is associated in serum IgE regulation or asthma we genotyped the SNPs in a Finnish asthmatic founder population. The association analyses were made using haplotype pattern mining. RESULTS: Fifteen novel SNPs were found in the T-bet gene. Within the Finnish asthmatic founder population, there was no association between T-bet SNPs and high serum IgE level or asthma. CONCLUSIONS: The genetic variability in the T-bet gene does not play a role in the pathogenesis of human asthma. Our results provide a novel panel of SNPs in T-bet and will help determine whether the SNPs have a functional role in other T cell-mediated diseases.
OBJECTIVE: T-box expressed in T cells (T-bet) is a transcription factor regulating the commitment of T helper (Th) cells by driving the cells into the Th1 direction. Abnormal Th1/Th2 balance may lead to complex disorders like asthma or autoimmune diseases. Recent studies have suggested that T-bet might be a candidate gene for asthma. This led us to screen 23 Finnish individuals for single-nucleotide polymorphisms (SNPs) in the T-bet locus and study the association between the SNPs and high serum IgE level and asthma. METHODS: We screened all six exons, adjacent intronic areas and 2 kb of the 5'-flanking region from 23 individuals utilizing WAVE trade mark technology. To explore whether T-bet is associated in serum IgE regulation or asthma we genotyped the SNPs in a Finnish asthmatic founder population. The association analyses were made using haplotype pattern mining. RESULTS: Fifteen novel SNPs were found in the T-bet gene. Within the Finnish asthmatic founder population, there was no association between T-bet SNPs and high serum IgE level or asthma. CONCLUSIONS: The genetic variability in the T-bet gene does not play a role in the pathogenesis of humanasthma. Our results provide a novel panel of SNPs in T-bet and will help determine whether the SNPs have a functional role in other T cell-mediated diseases.
Authors: Jonne Vaarno; Emmi Ylikoski; Niko J Meltola; Juhani T Soini; Pekka Hänninen; Riitta Lahesmaa; Aleksi E Soini Journal: Nucleic Acids Res Date: 2004-07-19 Impact factor: 16.971
Authors: D Y Zhu; L F Jiang; X Z Deng; W Xiao; J P Pei; B J Li; C J Wang; J H Zhang; Q Zhang; Z X Zhou; W L Ding; X D Xu; M Yue Journal: Eur J Clin Microbiol Infect Dis Date: 2015-03-11 Impact factor: 3.267
Authors: Benjamin A Raby; Eun-Sook Hwang; Kristel Van Steen; Kelan Tantisira; Stanford Peng; Augusto Litonjua; Ross Lazarus; Cosmas Giallourakis; John D Rioux; David Sparrow; Edwin K Silverman; Laurie H Glimcher; Scott T Weiss Journal: Am J Respir Crit Care Med Date: 2005-09-22 Impact factor: 21.405
Authors: Pravitt Gourh; Sandeep K Agarwal; Dipal Divecha; Shervin Assassi; Gene Paz; Rajpreet K Arora-Singh; John D Reveille; Sanjay Shete; Maureen D Mayes; Frank C Arnett; Filemon K Tan Journal: Arthritis Rheum Date: 2009-12