Identification of iso- and n-propylphosphonates using liquid chromatography-tandem mass spectrometry and gas chromatography-Fourier transform infrared spectroscopy.

Organophosphorus nerve agents and their precursors, specifically listed in the schedules of chemicals in the Annex to the Chemical Weapons Convention (CWC), include analogues with C1-C3 alkyl groups on phosphorus. The Organisation for the Prohibition of Chemical Weapons (OPCW) requires designated laboratories to unequivocally identify isomeric propyl groups bonded to phosphorus in analytes that may be present in samples submitted for analysis. Homologous series of isomeric pairs of dialkyl iso- and n-propylphosphonates, alkyl iso- and n-propylphosphonochloridates, and alkyl iso- and n-propylphosphonofluoridates, have been analysed by liquid chromatography-ion trap tandem mass spectrometry and/or by gas chromatography-Fourier transform infrared spectroscopy. The results show that P-propyl isomers can be reliably differentiated by collision induced dissociation (CID) of selected fragment ions and by their infrared P=O stretching and C-H deformation frequencies.