Literature DB >> 15246659

Flower color modulations of Torenia hybrida by downregulation of chalcone synthase genes with RNA interference.

Ei-ichiro Fukusaki1, Kengo Kawasaki, Shin'ichiro Kajiyama, Chung-Il An, Kenichi Suzuki, Yoshikazu Tanaka, Akio Kobayashi.   

Abstract

Suppression of biosynthetic genes involved in flower color formation is an important approach for obtaining target flower colors. Here we report that flower color of the garden plant Torenia hybrida was successfully modulated by RNA interference (RNAi) against a gene of chalcone synthase (CHS), a key enzyme for anthocyanin and flavonoid biosynthesis. By using each of the coding region and the 3'-untranslated region of the CHS mRNA as an RNAi target, exhaustive and gene-specific gene silencing were successfully induced, and the original blue flower color was modulated to white and pale colors, respectively. Our results indicate that RNAi is quite useful for modulations of flower colors of commercially important garden plants.

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Year:  2004        PMID: 15246659     DOI: 10.1016/j.jbiotec.2004.02.019

Source DB:  PubMed          Journal:  J Biotechnol        ISSN: 0168-1656            Impact factor:   3.307


  30 in total

1.  Downregulation of putative UDP-glucose: flavonoid 3-O-glucosyltransferase gene alters flower coloring in Phalaenopsis.

Authors:  Wen-Huei Chen; Chi-Yin Hsu; Hao-Yun Cheng; Hsiang Chang; Hong-Hwa Chen; Mang-Jye Ger
Journal:  Plant Cell Rep       Date:  2011-01-28       Impact factor: 4.570

2.  Sequence variation of chalcone synthase gene in a spontaneous white-flower mutant of Chinese cabbage-pak-choi.

Authors:  Ming Jiang; Jiashu Cao
Journal:  Mol Biol Rep       Date:  2007-07-01       Impact factor: 2.316

3.  Simultaneous post-transcriptional gene silencing of two different chalcone synthase genes resulting in pure white flowers in the octoploid dahlia.

Authors:  Sho Ohno; Munetaka Hosokawa; Misa Kojima; Yoshikuni Kitamura; Atsushi Hoshino; Fumi Tatsuzawa; Motoaki Doi; Susumu Yazawa
Journal:  Planta       Date:  2011-06-18       Impact factor: 4.116

Review 4.  RNA interference: concept to reality in crop improvement.

Authors:  Satyajit Saurabh; Ambarish S Vidyarthi; Dinesh Prasad
Journal:  Planta       Date:  2014-01-09       Impact factor: 4.116

5.  Identification and characterization of genes induced for anthocyanin synthesis and chlorophyll degradation in regenerated torenia shoots using suppression subtractive hybridization, cDNA microarrays, and RNAi techniques.

Authors:  Yozo Nagira; Katsuyoshi Shimamura; Sayaka Hirai; Mamiko Shimanuki; Hiroaki Kodama; Yoshihiro Ozeki
Journal:  J Plant Res       Date:  2006-04-07       Impact factor: 2.629

6.  RNA interference-based gene silencing as an efficient tool for functional genomics in hexaploid bread wheat.

Authors:  Silvia Travella; Theres E Klimm; Beat Keller
Journal:  Plant Physiol       Date:  2006-07-21       Impact factor: 8.340

7.  Transcriptional activation of anthocyanin structural genes in Torenia 'Kauai Rose' via overexpression of anthocyanin regulatory transcription factors.

Authors:  Xu Junping; Aung Htay Naing; Chang Kil Kim
Journal:  3 Biotech       Date:  2018-11-10       Impact factor: 2.406

8.  Enhancers of Agrobacterium-mediated Transformation of Tibouchina semidecandra Selected on the Basis of GFP Expression.

Authors:  Wilson Thau Lym Yong; Erle Stanley Henry; Janna Ong Abdullah
Journal:  Trop Life Sci Res       Date:  2010-12

9.  Functional analyses of a flavonol synthase-like gene from Camellia nitidissima reveal its roles in flavonoid metabolism during floral pigmentation.

Authors:  Xing-Wen Zhou; Zheng-Qi Fan; Yue Chen; Yu-Lin Zhu; Ji-Yuan Li; Heng-Fu Yin
Journal:  J Biosci       Date:  2013-09       Impact factor: 1.826

10.  Production of red-flowered plants by genetic engineering of multiple flavonoid biosynthetic genes.

Authors:  Takashi Nakatsuka; Yoshiko Abe; Yuko Kakizaki; Saburo Yamamura; Masahiro Nishihara
Journal:  Plant Cell Rep       Date:  2007-07-17       Impact factor: 4.570

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