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Literature DB >> 15243800

The usefulness of short-term in vitro cultivation for the detection and molecular study of Blastocystis hominis in stool specimens.

Sumeth Termmathurapoj¹, Saovanee Leelayoova, Pote Aimpun, Umaporn Thathaisong, Thirayost Nimmanon, Paanjit Taamasri, Mathirut Mungthin.

Abstract

When in vitro cultivation was used as the 'gold standard' for the detection of Blastocystis hominis in stool specimens, simple smear and trichrome staining showed sensitivities of 16.7% and 40.2% and specificities of 94% and 80.4%, respectively. In vitro cultivation also enhanced PCR amplification for the detection of B. hominis in stool specimens. Our data show the usefulness of in vitro cultivation for the detection and molecular study of B. hominis in stool specimens.

Entities: Species

Mesh：

Substances：
DNA, Protozoan

Year: 2004 PMID： 15243800 DOI： 10.1007/s00436-004-1157-x

Source DB: PubMed Journal: Parasitol Res ISSN： 0932-0113 Impact factor: 2.289

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Authors: R W Ashford; E A Atkinson
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Review 5. New insights on classification, identification, and clinical relevance of Blastocystis spp.

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