| Literature DB >> 15242755 |
Bert de Groef1, Sylvia V H Grommen, Inge Mertens, Liliane Schoofs, Eduard R Kühn, Veerle M Darras.
Abstract
We report the cloning of the complete coding sequence of the putative chicken type 2 corticotropin-releasing hormone receptor (CRH-R2) by rapid amplification of cDNA ends (RACE). The chicken CRH-R2 is a 412-amino acid 7-transmembrane G protein-coupled receptor, showing 87% identity to the Xenopus laevis and Oncorhynchus keta CRH-R2s, and 78-80% to mammalian CRH-R2s. The distribution of CRH-R2 mRNA was studied by RT-PCR analysis and compared to CRH-R1 distribution. Both CRH-R1 and CRH-R2 mRNA are expressed in the main chicken brain parts. In peripheral organs, CRH-R1 mRNA shows a more restricted distribution, whereas CRH-R2 mRNA is expressed in every tissue investigated, indicating that a number of actions of CRH and/or CRH-like peptides remain to be discovered in the chicken as well as in other vertebrates.Entities:
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Year: 2004 PMID： 15242755 DOI： 10.1016/j.ygcen.2004.05.006
Source DB: PubMed Journal: Gen Comp Endocrinol ISSN： 0016-6480 Impact factor: 2.822