Literature DB >> 15240823

A facile method for high-throughput co-expression of protein pairs.

Andrei Alexandrov1, Marissa Vignali, Douglas J LaCount, Erin Quartley, Christina de Vries, Daniela De Rosa, Julie Babulski, Sarah F Mitchell, Lori W Schoenfeld, Stanley Fields, Wim G Hol, Mark E Dumont, Eric M Phizicky, Elizabeth J Grayhack.   

Abstract

We developed a method to co-express protein pairs from collections of otherwise identical Escherichia coli plasmids expressing different ORFs by incorporating a 61-nucleotide sequence (LINK) into the plasmid to allow generation of tandem plasmids. Tandem plasmids are formed in a ligation-independent manner, propagate efficiently, and produce protein pairs in high quantities. This greatly facilitates co-expression for structural genomics projects that produce thousands of clones bearing identical origins and antibiotic markers.

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Year:  2004        PMID： 15240823     DOI： 10.1074/mcp.T400008-MCP200

Source DB:  PubMed          Journal:  Mol Cell Proteomics        ISSN： 1535-9476            Impact factor:   5.911


  71 in total

1.  A domain of the actin binding protein Abp140 is the yeast methyltransferase responsible for 3-methylcytidine modification in the tRNA anti-codon loop.

Authors:  Sonia D'Silva; Steffen J Haider; Eric M Phizicky
Journal:  RNA       Date:  2011-04-25       Impact factor: 4.942

2.  Structure determination of glycogen synthase kinase-3 from Leishmania major and comparative inhibitor structure-activity relationships with Trypanosoma brucei GSK-3.

Authors:  Kayode K Ojo; Tracy L Arakaki; Alberto J Napuli; Krishna K Inampudi; Katelyn R Keyloun; Li Zhang; Wim G J Hol; Christophe L M J Verlinde; Ethan A Merritt; Wesley C Van Voorhis
Journal:  Mol Biochem Parasitol       Date:  2010-12-30       Impact factor: 1.759

3.  Cap-domain closure enables diverse substrate recognition by the C2-type haloacid dehalogenase-like sugar phosphatase Plasmodium falciparum HAD1.

Authors:  Jooyoung Park; Ann M Guggisberg; Audrey R Odom; Niraj H Tolia
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  2015-08-25

4.  Expression of membrane proteins from Mycobacterium tuberculosis in Escherichia coli as fusions with maltose binding protein.

Authors:  A Korepanova; J D Moore; H B Nguyen; Y Hua; T A Cross; F Gao
Journal:  Protein Expr Purif       Date:  2006-12-24       Impact factor: 1.650

5.  Control of translation efficiency in yeast by codon-anticodon interactions.

Authors:  Daniel P Letzring; Kimberly M Dean; Elizabeth J Grayhack
Journal:  RNA       Date:  2010-10-22       Impact factor: 4.942

6.  Structure of Lmaj006129AAA, a hypothetical protein from Leishmania major.

Authors:  Tracy Arakaki; Isolde Le Trong; Eric Phizicky; Erin Quartley; George DeTitta; Joseph Luft; Angela Lauricella; Lori Anderson; Oleksandr Kalyuzhniy; Elizabeth Worthey; Peter J Myler; David Kim; David Baker; Wim G J Hol; Ethan A Merritt
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2006-02-24

7.  The Toxoplasma gondii centrosome is the platform for internal daughter budding as revealed by a Nek1 kinase mutant.

Authors:  Chun-Ti Chen; Marc-Jan Gubbels
Journal:  J Cell Sci       Date:  2013-05-31       Impact factor: 5.285

8.  The Toxoplasma gondii kinetochore is required for centrosome association with the centrocone (spindle pole).

Authors:  Megan Farrell; Marc-Jan Gubbels
Journal:  Cell Microbiol       Date:  2013-09-10       Impact factor: 3.715

9.  tRNA m7G methyltransferase Trm8p/Trm82p: evidence linking activity to a growth phenotype and implicating Trm82p in maintaining levels of active Trm8p.

Authors:  Andrei Alexandrov; Elizabeth J Grayhack; Eric M Phizicky
Journal:  RNA       Date:  2005-04-05       Impact factor: 4.942

10.  Functional fusions of T4 lysozyme in the third intracellular loop of a G protein-coupled receptor identified by a random screening approach in yeast.

Authors:  Elizabeth Mathew; Fa-Xiang Ding; Fred Naider; Mark E Dumont
Journal:  Protein Eng Des Sel       Date:  2012-10-16       Impact factor: 1.650
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