Literature DB >> 15240249

Thioesterase II of Escherichia coli plays an important role in 3-hydroxydecanoic acid production.

Zhong Zheng1, Qiang Gong, Tao Liu, Ying Deng, Jin-Chun Chen, Guo-Qiang Chen.   

Abstract

3-Hydroxydecanoic acid (3HD) was produced in Escherichia coli by mobilizing (R)-3-hydroxydecanoyl-acyl carrier protein-coenzyme A transacylase (PhaG, encoded by the phaG gene). By employing an isogenic tesB (encoding thioesterase II)-negative knockout E. coli strain, CH01, it was found that the expressions of tesB and phaG can up-regulate each other. In addition, 3HD was synthesized from glucose or fructose by recombinant E. coli harboring phaG and tesB. This study supports the hypothesis that the physiological role of thioesterase II in E. coli is to prevent the abnormal accumulation of intracellular acyl-coenzyme A.

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Year:  2004        PMID: 15240249      PMCID: PMC444760          DOI: 10.1128/AEM.70.7.3807-3813.2004

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN: 0099-2240            Impact factor:   4.792


  27 in total

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6.  Crystallization of the acyl-CoA thioesterase TesB from Yersinia pestis.

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