| Literature DB >> 1523879 |
C Martínez1, K Dalsgaard, J A López de Turiso, E Cortés, C Vela, J I Casal.
Abstract
The VP2 gene of porcine parvovirus was cloned in the baculovirus system and expressed in insect cells. The resulting product was present in high yield. It self-assembled into particles which were structurally and antigenically indistinguishable from regular PPV capsids. A high degree of purity of the recombinant capsids was obtained by ammonium sulphate precipitation of cell lysates. These virus-like particles were used as antigen in the immunization of two pigs. The pigs elicited an immune response which, when assayed by standard serological techniques, was identical to that of a commercial vaccine. The amount of recombinant antigen needed in a vaccine dose was only 3 micrograms in a primary dose and 1.5 micrograms in the booster.Entities:
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Year: 1992 PMID： 1523879 DOI： 10.1016/0264-410x(92)90090-7
Source DB: PubMed Journal: Vaccine ISSN： 0264-410X Impact factor: 3.641