Literature DB >> 15237978

Pyrene-labeled oligodeoxynucleotide probe for detecting base insertion by excimer fluorescence emission.

Akimitsu Okamoto1, Tomohisa Ichiba, Isao Saito.   

Abstract

Novel ODN probes for insertion polymorphism detection have been devised. The ODN probes labeled with two pyrene chromophores emit strong excimer fluorescence when hybridized with DNA containing one inserted base. This method is useful for the determination of the presence/absence of insertion polymorphisms located at a specific site on the target DNA.

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Year:  2004        PMID: 15237978     DOI: 10.1021/ja049061d

Source DB:  PubMed          Journal:  J Am Chem Soc        ISSN: 0002-7863            Impact factor:   15.419


  9 in total

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