OBJECTIVES: This study was performed to examine the cause of the increase in quinolone-resistant Neisseria gonorrhoeae (QRNG) observed in Korea. METHODS: The antimicrobial susceptibilities of 190 isolates of gonococci from Korea in 2000 were examined by NCCLS methods, and subsets of these isolates underwent mutation analysis of the quinolone resistance-determining regions (QRDRs) of gyrA and parC. Molecular epidemiological characterization of 25 Korean isolates and 54 isolates from overseas was performed by pulsed-field gel electrophoresis (PFGE) and the results compared. RESULTS: Most (172, 90.5%) of the 190 gonococci tested displayed reduced susceptibility to ciprofloxacin. All strains with high-level ciprofloxacin resistance (ciprofloxacin MIC >/= 4 mg/L) contained a double amino acid alteration at the 91 and 95 positions in the QRDR of GyrA and a single alteration in ParC. PFGE types of high-level QRNG in Korea were mostly different from those of other nearby countries. CONCLUSIONS: These results suggest that the observed increase in ciprofloxacin-resistant isolates is due to the mutation and spread of Korean multiclonal isolates rather than importation from overseas.
OBJECTIVES: This study was performed to examine the cause of the increase in quinolone-resistant Neisseria gonorrhoeae (QRNG) observed in Korea. METHODS: The antimicrobial susceptibilities of 190 isolates of gonococci from Korea in 2000 were examined by NCCLS methods, and subsets of these isolates underwent mutation analysis of the quinolone resistance-determining regions (QRDRs) of gyrA and parC. Molecular epidemiological characterization of 25 Korean isolates and 54 isolates from overseas was performed by pulsed-field gel electrophoresis (PFGE) and the results compared. RESULTS: Most (172, 90.5%) of the 190 gonococci tested displayed reduced susceptibility to ciprofloxacin. All strains with high-level ciprofloxacin resistance (ciprofloxacin MIC >/= 4 mg/L) contained a double amino acid alteration at the 91 and 95 positions in the QRDR of GyrA and a single alteration in ParC. PFGE types of high-level QRNG in Korea were mostly different from those of other nearby countries. CONCLUSIONS: These results suggest that the observed increase in ciprofloxacin-resistant isolates is due to the mutation and spread of Korean multiclonal isolates rather than importation from overseas.
Authors: Supriya D Mehta; Ian Maclean; Jeckoniah O Ndinya-Achola; Stephen Moses; Irene Martin; Allan Ronald; Lawrence Agunda; Ruth Murugu; Robert C Bailey; Johan Melendez; Jonathan M Zenilman Journal: Antimicrob Agents Chemother Date: 2011-05-23 Impact factor: 5.191
Authors: Aline A Uehara; Efigênia L T Amorin; Maria de Fátima Ferreira; Claudia F Andrade; Maysa B M Clementino; Ivano de Filippis; Felipe P G Neves; Tatiana de C A Pinto; Lúcia M Teixeira; Marcia Giambiagi-Demarval; Sérgio E L Fracalanzza Journal: J Clin Microbiol Date: 2011-10-05 Impact factor: 5.948
Authors: David M Whiley; E Athena Limnios; Sanghamitra Ray; Theo P Sloots; John W Tapsall Journal: Antimicrob Agents Chemother Date: 2007-06-25 Impact factor: 5.191
Authors: Meklit Workneh; Matthew M Hamill; Francis Kakooza; Emmanuel Mande; Jessica Wagner; Olive Mbabazi; Rodney Mugasha; Henry Kajumbula; Richard Walwema; Jonathan Zenilman; Patrick Musinguzi; Peter Kyambadde; Mohammed Lamorde; Yukari C Manabe Journal: JMIR Public Health Surveill Date: 2020-06-10