Yun-Hong Li1, Chen Wang, Kui Meng, Long-Bang Chen, Xiao-Jun Zhou. 1. Department of Gastroenterology, Gulou Hospital, Medical College of Nanjing University, 321 Zhongshan Road, Nanjing 210008, Jiangsu Province, China. liyunhong37@vip.sina.com.cn
Abstract
AIM: To evaluate the role of survivin and caspase-3 in apoptosis of gastric carcinoma, as well as in prognosis of patients with gastric carcinoma. METHODS: Expressions of survivin and caspase-3 were investigated immunohistochemically in 80 gastric carcinoma patients without a history of chemo-radiation therapy. Tumor cell apoptosis was examined by TUNEL method. RESULTS: Immunohistochemical analysis showed that survivin expression was positive in 61 of 80 patients (76%) with gastric carcinoma. In contrast, no expression of survivin in adjacent normal tissues was detected. Expression level of caspase-3 was higher in normal tissues than in carcinoma. Patients with higher expression of survivin had worse histological grades and pathological stages. Expression of caspase-3 was significantly associated with histological stages, but not with the pathological stages. Although survivin expression in carcinoma was not inversely related to caspase-3, patients with survivin (-) and caspase-3(+) had the maximum apoptosis index. CONCLUSION: Expression level of survivin was associated with histological grades and pathological stages of the tumor, indicating that survivin may be a poor prognosis factor for gastric carcinoma. Unlike caspase-3, survivin (an apoptosis inhibitor) can markedly inhibit the apoptosis of tumor cells.
AIM: To evaluate the role of survivin and caspase-3 in apoptosis of gastric carcinoma, as well as in prognosis of patients with gastric carcinoma. METHODS: Expressions of survivin and caspase-3 were investigated immunohistochemically in 80 gastric carcinomapatients without a history of chemo-radiation therapy. Tumor cell apoptosis was examined by TUNEL method. RESULTS: Immunohistochemical analysis showed that survivin expression was positive in 61 of 80 patients (76%) with gastric carcinoma. In contrast, no expression of survivin in adjacent normal tissues was detected. Expression level of caspase-3 was higher in normal tissues than in carcinoma. Patients with higher expression of survivin had worse histological grades and pathological stages. Expression of caspase-3 was significantly associated with histological stages, but not with the pathological stages. Although survivin expression in carcinoma was not inversely related to caspase-3, patients with survivin (-) and caspase-3(+) had the maximum apoptosis index. CONCLUSION: Expression level of survivin was associated with histological grades and pathological stages of the tumor, indicating that survivin may be a poor prognosis factor for gastric carcinoma. Unlike caspase-3, survivin (an apoptosis inhibitor) can markedly inhibit the apoptosis of tumor cells.
Authors: C Sun; M Cai; A H Gunasekera; R P Meadows; H Wang; J Chen; H Zhang; W Wu; N Xu; S C Ng; S W Fesik Journal: Nature Date: 1999-10-21 Impact factor: 49.962
Authors: C S Duckett; V E Nava; R W Gedrich; R J Clem; J L Van Dongen; M C Gilfillan; H Shiels; J M Hardwick; C B Thompson Journal: EMBO J Date: 1996-06-03 Impact factor: 11.598
Authors: C Adida; C Haioun; P Gaulard; E Lepage; P Morel; J Briere; H Dombret; F Reyes; J Diebold; C Gisselbrecht; G Salles; D C Altieri; T J Molina Journal: Blood Date: 2000-09-01 Impact factor: 22.113