AIM: To prospectively assess the sensitivity, specificity and time to positivity of theUltra-rapid urease test (URUT) for Helicobacter pylori (H pylori), and compare the results of one with those of two biopsies. METHODS: Five antral biopsies were taken in consecutive patients undergoing upper endoscopy: one and two biopsies for URUT, and one each for H pylori culture and histology. URUT was read at 1, 5, 10, 20 and 30 min, 1, 2, 3 and 24 h after biopsy insertion into the reagent. A positive histology and/or culture was used as positive reference "gold standards". RESULTS: URUT was more sensitive for detecting H pylori with two biopsies rather than one, at all time points up to 120 min. The sensitivity improved from 3.6% to 82.1% for one biopsy and 10.7% to 85.7% for two biopsies from 1 to 120 min. The sensitivity reached 96.4% at 24 h for both, but the specificity reduced from 100% to 96% and 92% for one and two biopsies, respectively. CONCLUSION: Development of a positive URUT result is hastened by doubling the number of gastric biopsies. We recommend taking two instead of one biopsy to achieve an earlier positive URUT result so that H pylori eradication therapy can be initiated before patient is discharged from the endoscopy suite.
AIM: To prospectively assess the sensitivity, specificity and time to positivity of theUltra-rapid urease test (URUT) for Helicobacter pylori (H pylori), and compare the results of one with those of two biopsies. METHODS: Five antral biopsies were taken in consecutive patients undergoing upper endoscopy: one and two biopsies for URUT, and one each for H pylori culture and histology. URUT was read at 1, 5, 10, 20 and 30 min, 1, 2, 3 and 24 h after biopsy insertion into the reagent. A positive histology and/or culture was used as positive reference "gold standards". RESULTS: URUT was more sensitive for detecting H pylori with two biopsies rather than one, at all time points up to 120 min. The sensitivity improved from 3.6% to 82.1% for one biopsy and 10.7% to 85.7% for two biopsies from 1 to 120 min. The sensitivity reached 96.4% at 24 h for both, but the specificity reduced from 100% to 96% and 92% for one and two biopsies, respectively. CONCLUSION: Development of a positive URUT result is hastened by doubling the number of gastric biopsies. We recommend taking two instead of one biopsy to achieve an earlier positive URUT result so that H pylori eradication therapy can be initiated before patient is discharged from the endoscopy suite.
Authors: J Y Kang; K G Yeoh; K Y Ho; R Guan; T P Lim; S H Quak; A Wee; D Teo; Y W Ong Journal: J Gastroenterol Hepatol Date: 1997-10 Impact factor: 4.029
Authors: B C Wong; W M Wong; W H Wang; V S Tang; J Young; K C Lai; S T Yuen; S Y Leung; W H Hu; C K Chan; W M Hui; S K Lam Journal: Aliment Pharmacol Ther Date: 2001-04 Impact factor: 8.171
Authors: Jesús Saez; Sofía Belda; Miguel Santibáñez; Juan Carlos Rodríguez; Javier Sola-Vera; Antonio Galiana; Montserrat Ruiz-García; Alicia Brotons; Elena López-Girona; Eva Girona; Carlos Sillero; Gloria Royo Journal: J Clin Microbiol Date: 2012-07-25 Impact factor: 5.948