Literature DB >> 15221461

Detection of circulating antigens in experimental anisakiasis by two-site enzyme-linked immunosorbent assay.

M Campos1, L Martín, V Díaz, I Mañas, B Morales, J Lozano.   

Abstract

A two-site enzyme-linked immunosorbent assay (ELISA) based on the avidin-biotin system was developed to study circulating antigens in the sera of rats orally or intraperitoneally infected with L3 larvae of Anisakis simplex s.l. Somatic and excretory-secretory antigens were detected from 24 h post-infection in all infected rats. A direct relationship between the number of larvae inoculated and the concentration of antigens in the sera was demonstrated; however, the amount of antigen decreased with the course of the infection. Comparative analysis revealed that intraperitoneal inoculation is most appropriate for detecting excretory-secretory antigens in serum. The minimum concentrations of A. simplex antigen detectable were approximately 2.5 microg/ml for somatic and excretory-secretory antigens. This antigen detection assay may have application in the diagnosis of anisakiasis.

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Year:  2004        PMID： 15221461     DOI： 10.1007/s00436-004-1138-0

Source DB:  PubMed          Journal:  Parasitol Res        ISSN： 0932-0113            Impact factor:   2.289


  12 in total

1.  Laboratory determinations in Anisakis simplex allergy.

Authors:  M D del Pozo; I Moneo; L F de Corres; M T Audicana; D Muñoz; E Fernandez; J A Navarro; M García
Journal:  J Allergy Clin Immunol       Date:  1996-04       Impact factor: 10.793

2.  Protein measurement with the Folin phenol reagent.

Authors:  O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal:  J Biol Chem       Date:  1951-11       Impact factor: 5.157

3.  Allergenic cross-reactivity between third stage larvae of Hysterothylacium aduncum and Anisakis simplex.

Authors:  E Fernández-Caldas; S Quirce; F Marañón; M L Diez Gómez; H Gijón Botella; R López Román
Journal:  J Allergy Clin Immunol       Date:  1998-04       Impact factor: 10.793

4.  Antigenic cross-reactivity in mice between third-stage larvae of Anisakis simplex and other nematodes.

Authors:  R Iglesias; J Leiro; F M Ubeira; M T Santamarina; I Navarrete; M L Sanmartín
Journal:  Parasitol Res       Date:  1996       Impact factor: 2.289

5.  The secreted and somatic antigens of the third stage larva of Anisakis simplex, and antigenic relationship with Ascaris suum, Ascaris lumbricoides, and Toxocara canis.

Authors:  M W Kennedy; J Tierney; P Ye; F A McMonagle; A McIntosh; D McLaughlin; J W Smith
Journal:  Mol Biochem Parasitol       Date:  1988-10       Impact factor: 1.759

6.  Some factors which influence the in vitro maintenance of Anisakis simplex (Nematoda).

Authors:  L Iglesias; A Valero; F J Adroher
Journal:  Folia Parasitol (Praha)       Date:  1997       Impact factor: 2.122

7.  Anisakis simplex: antigen recognition and antibody production in experimentally infected mice.

Authors:  R Iglesias; J Leiro; F M Ubeira; M T Santamarina; M L Sanmartín
Journal:  Parasite Immunol       Date:  1993-05       Impact factor: 2.280

8.  Determination of IgE in the serum of patients with allergic reactions to four species of fish-parasite anisakids.

Authors:  A Valero; S Terrados; V Díaz; V Reguera; J Lozano
Journal:  J Investig Allergol Clin Immunol       Date:  2003       Impact factor: 4.333

9.  A serodiagnostic assay by microenzyme-linked immunosorbent assay for human anisakiasis using a monoclonal antibody specific for Anisakis larvae antigen.

Authors:  A Yagihashi; N Sato; S Takahashi; H Ishikura; K Kikuchi
Journal:  J Infect Dis       Date:  1990-05       Impact factor: 5.226

10.  Quantitative determination of circulating antigens in human schistosomiasis mansoni using an indirect hemagglutination assay.

Authors:  A M Deelder; N De Jonge; Y E Fillié; D Kornelis; D Helaha; Z L Qian; P De Caluwé; A M Polderman
Journal:  Am J Trop Med Hyg       Date:  1989-01       Impact factor: 2.345
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  1 in total

1.  A rat model of intragastric infection with Anisakis spp. live larvae: histopathological study.

Authors:  Jaime Zuloaga; Cruz Rodríguez-Bobada; María Teresa Corcuera; Fernando Gómez-Aguado; Pablo González; Rosa Rodríguez-Perez; Javier Arias-Díaz; María Luisa Caballero
Journal:  Parasitol Res       Date:  2013-02-23       Impact factor: 2.289
  1 in total

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