| Literature DB >> 15220082 |
Iben Damager1, Shin Numao, Hongming Chen, Gary D Brayer, Stephen G Withers.
Abstract
Derivatives of maltose and maltotriose were chemically synthesised as substrates for human pancreatic alpha-amylases and subjected to kinetic analysis. Rates measured were shown to reflect both hydrolysis and transglycosylation reactions. 4-O-Methylated derivatives of these substrates underwent only hydrolysis, thereby simplifying kinetic analyses. These modified substrates may be used for the detection and kinetic analysis of alpha-amylases, and are useful in rapidly screening for novel alpha-amylase inhibitors and for subsequent kinetic characterisation.Entities:
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Year: 2004 PMID： 15220082 DOI： 10.1016/j.carres.2004.05.019
Source DB: PubMed Journal: Carbohydr Res ISSN： 0008-6215 Impact factor: 2.104