| Literature DB >> 15219832 |
Rui Vitorino1, Maria João C Lobo, José Duarte, António J Ferrer-Correia, Kenneth B Tomer, Joshua R Dubin, Pedro M Domingues, Francisco M L Amado.
Abstract
In spite of the present knowledge about saliva components and their respective functions, the mechanism(s) of pellicle and dental plaque formation have hitherto remained obscure. This has prompted recent efforts on in vitro studies using hydroxyapatite (HA) as an enamel model. In the present study salivary proteins adsorbed to HA were extracted with TFA and EDTA and resolved by 2D electrophoresis over a pH range between 3 and 10, digested, and then analysed by MALDI-TOF/TOF mass spectrometry and tandem mass spectrometry. Nineteen different proteins were identified using automated MS and MS/MS data acquisition. Among them, cystatins, amylase, carbonic anhydrase, and calgranulin B, were identified.Entities:
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Year: 2004 PMID： 15219832 DOI： 10.1016/j.bbrc.2004.05.169
Source DB: PubMed Journal: Biochem Biophys Res Commun ISSN： 0006-291X Impact factor: 3.575