PURPOSE: Positive associations between the presence of micrometastatic tumor cells and disease aggressiveness have been reported in several tumor types, but the clinical implications are still not established. We wanted to test a new, sensitive immunomagnetic detection method on bone marrow (BM) and peripheral blood (PB) samples from patients with malignant melanoma and relate the findings to clinical outcome. EXPERIMENTAL DESIGN: Samples from 210 patients admitted for relapse of cutaneous melanoma were examined. Mononuclear cell fractions isolated from BM and PB were incubated with superparamagnetic particles coated with antimelanoma antibodies. Live tumor cells with bound beads were isolated with a magnet and identified in a microscope as cell-bead rosettes. Beads without antibody or with an irrelevant antibody were used as controls. The whole procedure was completed within 2-3 h. The identity of the cells was confirmed with a new double labeling procedure with fluorescent microparticles. RESULTS: Rosetted melanoma cells were found in BM aspirates of 35 of 186 (19%) patients, but in only 2 of 208 (1%) PB samples. The controls were all negative. After a median observation time of 1.1 year (range, 0-6.8 years), patients with tumor cells in BM showed a significantly shorter overall survival from time of BM aspiration (P = 0.009). In multiple regression analysis, a positive BM test was a strong indicator of overall survival (P = 0.021), associated with disease stage (American Joint Committee on Cancer) and with the number of metastatic sites, but not with the primary (Breslow) tumor depth and morphology. CONCLUSIONS: The results demonstrate the prognostic significance of detecting BM micrometastasis in melanoma patients. The results strengthen the validity of the immunobead technique. In contrast to other techniques, the method identifies intact, live tumor cells that can be further characterized, making the assay attractive for extended use.
PURPOSE: Positive associations between the presence of micrometastatic tumor cells and disease aggressiveness have been reported in several tumor types, but the clinical implications are still not established. We wanted to test a new, sensitive immunomagnetic detection method on bone marrow (BM) and peripheral blood (PB) samples from patients with malignant melanoma and relate the findings to clinical outcome. EXPERIMENTAL DESIGN: Samples from 210 patients admitted for relapse of cutaneous melanoma were examined. Mononuclear cell fractions isolated from BM and PB were incubated with superparamagnetic particles coated with antimelanoma antibodies. Live tumor cells with bound beads were isolated with a magnet and identified in a microscope as cell-bead rosettes. Beads without antibody or with an irrelevant antibody were used as controls. The whole procedure was completed within 2-3 h. The identity of the cells was confirmed with a new double labeling procedure with fluorescent microparticles. RESULTS: Rosetted melanoma cells were found in BM aspirates of 35 of 186 (19%) patients, but in only 2 of 208 (1%) PB samples. The controls were all negative. After a median observation time of 1.1 year (range, 0-6.8 years), patients with tumor cells in BM showed a significantly shorter overall survival from time of BM aspiration (P = 0.009). In multiple regression analysis, a positive BM test was a strong indicator of overall survival (P = 0.021), associated with disease stage (American Joint Committee on Cancer) and with the number of metastatic sites, but not with the primary (Breslow) tumor depth and morphology. CONCLUSIONS: The results demonstrate the prognostic significance of detecting BM micrometastasis in melanomapatients. The results strengthen the validity of the immunobead technique. In contrast to other techniques, the method identifies intact, live tumor cells that can be further characterized, making the assay attractive for extended use.
Authors: Jianbo Yang; Matthew A Price; Leah E C Wanshura; Jinsong He; Mei Yi; Danny R Welch; Guiyuan Li; Sean Conner; Jonathan Sachs; Eva A Turley; James B McCarthy Journal: Melanoma Res Date: 2019-08 Impact factor: 3.599
Authors: Elin S Gray; Anna L Reid; Samantha Bowyer; Leslie Calapre; Kelvin Siew; Robert Pearce; Lester Cowell; Markus H Frank; Michael Millward; Mel Ziman Journal: J Invest Dermatol Date: 2015-04-01 Impact factor: 8.551
Authors: Sara R Bang-Christensen; Rasmus S Pedersen; Marina A Pereira; Thomas M Clausen; Caroline Løppke; Nicolai T Sand; Theresa D Ahrens; Amalie M Jørgensen; Yi Chieh Lim; Louise Goksøyr; Swati Choudhary; Tobias Gustavsson; Robert Dagil; Mads Daugaard; Adam F Sander; Mathias H Torp; Max Søgaard; Thor G Theander; Olga Østrup; Ulrik Lassen; Petra Hamerlik; Ali Salanti; Mette Ø Agerbæk Journal: Cells Date: 2019-08-28 Impact factor: 6.600
Authors: Jonathan J Cools-Lartigue; Cristin S McCauley; Jean-Claude A Marshall; Sebastian Di Cesare; Francois Gregoire; Emilia Antecka; Patrick Logan; Miguel N Burnier Journal: Mol Vis Date: 2008-01-10 Impact factor: 2.367
Authors: A K Rud; E Borgen; G M Mælandsmo; K Flatmark; H Le; D Josefsen; I Solvoll; C B Schirmer; Å Helland; L Jørgensen; O T Brustugun; Ø Fodstad; K Boye Journal: Br J Cancer Date: 2013-08-13 Impact factor: 7.640