| Literature DB >> 15217319 |
Yasuo Fukutomi1, Masanori Matsuoka, Fumishige Minagawa, Satoshi Toratani, Gregory McCormick, James Krahenbuhl.
Abstract
In these studies, metabolically active Mycobacterium leprae were maintained for as long as 8 weeks in monolayer cultures of mouse peritoneal macrophages (MPhi). Supplemental IL-10, but not TGF-beta, bolstered, directly or indirectly, M. leprae metabolism in mouse MPhi. In the cell culture system temperature setting is extremely important and 31 to 33 degrees C incubation temperature was more permissive than 37 degrees C. Acid fast staining and transmission electron microscopy (TEM) of intracellular M. leprae revealed visible elongation of bacilli cultured under the above ideal conditions.Entities:
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Year: 2004 PMID： 15217319 DOI： 10.1489/1544-581X(2004)072<0016:ITOMBI>2.0.CO;2
Source DB: PubMed Journal: Int J Lepr Other Mycobact Dis ISSN： 0148-916X