Literature DB >> 15215606

The role of the DRY motif of human MC4R for receptor activation.

Yoshiaki Yamano1, Rio Kamon, Takao Yoshimizu, Yoshihisa Toda, Yuichi Oshida, Shigeyuki Chaki, Masanobu Yoshioka, Isao Morishima.   

Abstract

We constructed several mutant human MC4R cDNAs by site directed mutagenesis and expressed these receptors in COS-1 cells. The conserved DRY motif among GPCRs was mutated to generate eight mutants. While no MC4R ligand binding was detected in any of the mutants, one mutant, D146A, resulted in higher cAMP production in cells than the wild-type receptor without ligand stimulation.

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Year:  2004        PMID： 15215606     DOI： 10.1271/bbb.68.1369

Source DB:  PubMed          Journal:  Biosci Biotechnol Biochem        ISSN： 0916-8451            Impact factor:   2.043


  5 in total

1.  Mechanisms of melanocortin-2 receptor (MC2R) internalization and recycling in human embryonic kidney (hek) cells: identification of Key Ser/Thr (S/T) amino acids.

Authors:  Simon Roy; Sébastien Jean Roy; Sandra Pinard; Louis-Daniel Taillefer; Mohamed Rached; Jean-Luc Parent; Nicole Gallo-Payet
Journal:  Mol Endocrinol       Date:  2011-09-15

2.  Dynamic behavior of fully solvated beta2-adrenergic receptor, embedded in the membrane with bound agonist or antagonist.

Authors:  Peter Spijker; Nagarajan Vaidehi; Peter L Freddolino; Peter A J Hilbers; William A Goddard
Journal:  Proc Natl Acad Sci U S A       Date:  2006-03-21       Impact factor: 11.205

3.  In silico mutagenesis: a case study of the melanocortin 4 receptor.

Authors:  Yana Bromberg; John Overton; Christian Vaisse; Rudolph L Leibel; Burkhard Rost
Journal:  FASEB J       Date:  2009-05-05       Impact factor: 5.191

4.  Structure-activity relationships of cyclic lactam analogues of alpha-melanocyte-stimulating hormone (alpha-MSH) targeting the human melanocortin-3 receptor.

Authors:  Alexander V Mayorov; Minying Cai; Erin S Palmer; Matthew M Dedek; James P Cain; April R Van Scoy; Bahar Tan; Josef Vagner; Dev Trivedi; Victor J Hruby
Journal:  J Med Chem       Date:  2007-12-19       Impact factor: 7.446

5.  Insights from the analysis of conserved motifs and permitted amino acid exchanges in the human, the fly and the worm GPCR clusters.

Authors:  Balasubramanian Nagarathnam; Sankar Kannan; Varadhan Dharnidharka; Veluchamy Balakrishnan; Govindaraju Archunan; Ramanathan Sowdhamini
Journal:  Bioinformation       Date:  2011-08-20
  5 in total

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