Literature DB >> 15211037

Liquid-chromatography-coupled SAXS for accurate sizing of aggregating proteins.

Elizabeth Mathew1, Ahmed Mirza, Nick Menhart.   

Abstract

Small-angle X-ray scattering and size-exclusion chromatography have been combined within a unified experimental set-up to obtain molecular size information. Besides providing simultaneous corroborative data bearing on the same question from two distinct experimental techniques, passing the samples over a gel filtration column immediately prior to illumination by X-rays provides both a more homogeneous sample and a continuous set of data as the concentration is extrapolated to zero. This greatly facilitates analysis of data from oligomerizing or aggregating proteins and increases the reliability of the results.

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Year:  2004        PMID： 15211037     DOI： 10.1107/S0909049504014086

Source DB:  PubMed          Journal:  J Synchrotron Radiat        ISSN： 0909-0495            Impact factor:   2.616


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