Lei Li1, Jinyi Zhong. 1. Nutrition Institute, Medical College of Qingdao University, Qingdao 266021, China.
Abstract
OBJECTIVE: Investigating the effect of grape procyanidins(GPC) on the apoptosis and mitochondrial transmembrane potential of thymus cells damaged by H2O2. METHODS: Thymus cells of mouse were cultured in vitro. The cells were pretreated by GPC and then damaged by 125 mumol/L H2O2 for two hours. The levels of apoptosis and mitochondrial transmembrane potential were analysed by flow cytometry. RESULTS: Apoptosis rate and the level of mitochondrial transmembrane potential of positive group were (29.53 +/- 3.8)% and 27.24 +/- 2.2 (D value) respectively. After pretreatment of 50 mg/L GPC, the levels of apoptosis and mitochondrial transmembrane potential were (8.61 +/- 1.2)% and 87.55 +/- 4.7(D value). The differences between the two groups were significant (P < 0.05). CONCLUSION: GPC can inhibit apoptosis and decrease of mitochondrial transmembrane potential of thymus cells damaged by oxygen free radical.
OBJECTIVE: Investigating the effect of grape procyanidins(GPC) on the apoptosis and mitochondrial transmembrane potential of thymus cells damaged by H2O2. METHODS: Thymus cells of mouse were cultured in vitro. The cells were pretreated by GPC and then damaged by 125 mumol/L H2O2 for two hours. The levels of apoptosis and mitochondrial transmembrane potential were analysed by flow cytometry. RESULTS: Apoptosis rate and the level of mitochondrial transmembrane potential of positive group were (29.53 +/- 3.8)% and 27.24 +/- 2.2 (D value) respectively. After pretreatment of 50 mg/L GPC, the levels of apoptosis and mitochondrial transmembrane potential were (8.61 +/- 1.2)% and 87.55 +/- 4.7(D value). The differences between the two groups were significant (P < 0.05). CONCLUSION: GPC can inhibit apoptosis and decrease of mitochondrial transmembrane potential of thymus cells damaged by oxygen free radical.