| Literature DB >> 15207485 |
M Carmen Garcia-Pelayo1, Karina C Caimi, Jacqueline K Inwald, Jason Hinds, Fabiana Bigi, Maria I Romano, Dick van Soolingen, R Glyn Hewinson, Angel Cataldi, Stephen V Gordon.
Abstract
Mycobacterium microti is the agent of tuberculosis in wild voles and has been used as a live vaccine against tuberculosis in man and cattle. To explore the M. microti genome in greater detail, we used a M. tuberculosis H37Rv genomic DNA microarray to detect gene deletions among M. microti isolates. A number of deletions were identified that correlated with those described previously (Infect. Immun. 70 (2002) 5568) but a novel M. microti deletion was also found (MiD4) which removes 5 genes that code for ESAT-6 family antigens and PE-PPE proteins. Southern blot experiments showed that this region was also deleted from M. pinnipedii, a mycobacterium isolated from seals that is closely related to M. microti. Genes encoding ESAT-6 antigens and PE-PPE proteins appear to be frequently deleted from M. microti, and the implications of this are discussed.Entities:
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Year: 2004 PMID： 15207485 DOI： 10.1016/j.tube.2003.12.002
Source DB: PubMed Journal: Tuberculosis (Edinb) ISSN： 1472-9792 Impact factor: 3.131