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Literature DB >> 15205445

Independent regulation of two genes in Escherichia coli by tetracyclines and Tet repressor variants.

Annette Kamionka¹, Miriam Sehnal, Oliver Scholz, Wolfgang Hillen.

Abstract

We report a regulation system in Escherichia coli for independent regulation of two distinct reporter genes by application of Tet repressors with different specificities. One Tet repressor variant comprises wild-type tet operator (tetO) recognition and exclusive induction with the novel inducer 4-dedimethylamino-anhydrotetracycline. The other Tet repressor variant shows tetO-4C recognition and induction with tetracycline. We demonstrate that both variants are independently active in vivo and allow selective regulation of two genes in the same cell without any cross talk.

Entities: Chemical Disease Species

Mesh：

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Year: 2004 PMID： 15205445 PMCID： PMC421600 DOI： 10.1128/JB.186.13.4399-4401.2004

Source DB: PubMed Journal: J Bacteriol ISSN： 0021-9193 Impact factor: 3.490

18 in total

1. Regulated gene expression in Staphylococcus aureus for identifying conditional lethal phenotypes and antibiotic mode of action.

Authors: L Zhang; F Fan; L M Palmer; M A Lonetto; C Petit; L L Voelker; A St John; B Bankosky; M Rosenberg; D McDevitt
Journal: Gene Date: 2000-09-19 Impact factor: 3.688

2. Activity reversal of Tet repressor caused by single amino acid exchanges.

Authors: Oliver Scholz; Eva-Maria Henssler; Johannes Bail; Peter Schubert; Joanna Bogdanska-Urbaniak; Sabine Sopp; Marco Reich; Stefanie Wisshak; Martin Köstner; Ralph Bertram; Wolfgang Hillen
Journal: Mol Microbiol Date: 2004-08 Impact factor: 3.501

3. Determinants of protein-protein recognition by four helix bundles: changing the dimerization specificity of Tet repressor.

Authors: D Schnappinger; P Schubert; K Pfleiderer; W Hillen
Journal: EMBO J Date: 1998-01-15 Impact factor: 11.598

4. Mutations in the Tn10 tet repressor that interfere with induction. Location of the tetracycline-binding domain.

Authors: L D Smith; K P Bertrand
Journal: J Mol Biol Date: 1988-10-20 Impact factor: 5.469

5. Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoter.

Authors: L M Guzman; D Belin; M J Carson; J Beckwith
Journal: J Bacteriol Date: 1995-07 Impact factor: 3.490

6. Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements.

Authors: R Lutz; H Bujard
Journal: Nucleic Acids Res Date: 1997-03-15 Impact factor: 16.971

7. Quantitative analysis of Tn10 Tet repressor binding to a complete set of tet operator mutants.

Authors: C Sizemore; A Wissmann; U Gülland; W Hillen
Journal: Nucleic Acids Res Date: 1990-05-25 Impact factor: 16.971

8. A xylose-inducible Bacillus subtilis integration vector and its application.

Authors: L Kim; A Mogk; W Schumann
Journal: Gene Date: 1996-11-28 Impact factor: 3.688

9. Intracellular expression of Vitreoscilla hemoglobin (VHb) enhances total protein secretion and improves the production of alpha-amylase and neutral protease in Bacillus subtilis.

Authors: P T Kallio; J E Bailey
Journal: Biotechnol Prog Date: 1996 Jan-Feb

Independent regulation of two genes in Escherichia coli by tetracyclines and Tet repressor variants.

1. Regulated gene expression in Staphylococcus aureus for identifying conditional lethal phenotypes and antibiotic mode of action.

2. Activity reversal of Tet repressor caused by single amino acid exchanges.

3. Determinants of protein-protein recognition by four helix bundles: changing the dimerization specificity of Tet repressor.

4. Mutations in the Tn10 tet repressor that interfere with induction. Location of the tetracycline-binding domain.

5. Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoter.

6. Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements.

7. Quantitative analysis of Tn10 Tet repressor binding to a complete set of tet operator mutants.

8. A xylose-inducible Bacillus subtilis integration vector and its application.

9. Intracellular expression of Vitreoscilla hemoglobin (VHb) enhances total protein secretion and improves the production of alpha-amylase and neutral protease in Bacillus subtilis.

10. Studies on transformation of Escherichia coli with plasmids.

1. Tetracycline-dependent conditional gene knockout in Bacillus subtilis.

Review 2. The application of Tet repressor in prokaryotic gene regulation and expression.

3. Identifying ultrasensitive HGF dose-response functions in a 3D mammalian system for synthetic morphogenesis.

4. Synthetic biology tools for engineering Goodwin oscillation in Trypanosoma brucei brucei.

Review 5. Status quo of tet regulation in bacteria.