| Literature DB >> 15203218 |
Glenn K Fu1, Jonathan T Wang, Junming Yang, Janice Au-Young, Laura L Stuve.
Abstract
The rapid amplification of cDNA ends (RACE) procedure is a widely used PCR-based method to clone the cDNA ends of mRNA transcripts. Current RACE methods often produce a high background of nonspecific PCR products, which can exclude the identification of the target cDNA of interest. We describe here an improved RACE procedure using circular cDNA templates and demonstrate the successful extension cloning of 4406 cDNAs. Copyright 2004 Elsevier Inc.Mesh：
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Year: 2004 PMID： 15203218 DOI： 10.1016/j.ygeno.2004.01.011
Source DB: PubMed Journal: Genomics ISSN： 0888-7543 Impact factor: 5.736