| Literature DB >> 15188401 |
Stèphanie Huguet1, Valèrie Labas, Jean-Charles Duclos-Vallee, Arnaud Bruneel, Joëlle Vinh, Didier Samuel, Catherine Johanet, Eric Ballot.
Abstract
Our aim was to characterize antinuclear antibody (ANA) targets in type 1 autoimmune hepatitis (AIH1) using a proteomic tool. ANA-positive sera from 29 patients with AIH1 and 14 negative controls were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblotting. The principal antigens identified were a 30 kDa triplet band which was recognized by 79% of patient sera and 14% of control sera, and a 36 kDa antigen which was recognized by 52% and 14% of sera, respectively. The latter antigen was studied in more detail using two-dimensional electrophoresis (2-DE) and mass spectrometry (MS). Its identification corresponded to heterogeneous nuclear ribonucleoprotein A2/B1. This paper presents the first description of this protein as an antigen in AIH1.Entities:
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Year: 2004 PMID： 15188401 DOI： 10.1002/pmic.200300757
Source DB: PubMed Journal: Proteomics ISSN： 1615-9853 Impact factor: 3.984