Separation, purification, and characterization of polypeptide composition of subcomplexes of the main light-harvesting chlorophyll a/b-protein complex of photosystem II.

The protocols regarding separation and purification of LHCII subcomplexes by non-denaturing isoelectric focusing (ndIEF), confirmation of trimeric nature of the subcomplexes, and establishing their polypeptide composition are described below.