Literature DB >> 15186438

PCR detection of pathogenic viruses in southern California urban rivers.

S C Jiang1, W Chu.   

Abstract

AIMS: To investigate human viral contamination in urban rivers and its impact on coastal waters of southern California, USA. METHODS AND
RESULTS: Three types of human viruses (adeno, entero and hepatitis A) were detected using nested- and RT-PCR from 11 rivers and creeks. Faecal indicator bacteria as well as somatic and F-specific coliphage were also tested. Approximately 50% of the sites were positive for human adenoviruses. However, there was no clear relationship between detection of human viruses and the concentration of indicator bacteria and coliphage. Both faecal indicator bacteria and human viral input at beaches near river mouths were associated with storm events. The first storm of the wet season seemed to have the greatest impact on the quality of coastal water than following storm events.
CONCLUSIONS: This study provides the first direct evidence that human viruses are prevalent in southern California urban rivers. Urban run-off impacts coastal water quality most significantly during the storm season. SIGNIFICANCE AND IMPACT OF THE STUDY: To protect human health during water recreational activities, it is necessary to develop effective strategies to manage urban run-off during storm events.

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Year:  2004        PMID: 15186438     DOI: 10.1111/j.1365-2672.2004.02269.x

Source DB:  PubMed          Journal:  J Appl Microbiol        ISSN: 1364-5072            Impact factor:   3.772


  29 in total

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3.  Real-time PCR quantification of human adenoviruses in urban rivers indicates genome prevalence but low infectivity.

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Journal:  Appl Environ Microbiol       Date:  2005-11       Impact factor: 4.792

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10.  Presence of pathogens and indicator microbes at a non-point source subtropical recreational marine beach.

Authors:  Amir M Abdelzaher; Mary E Wright; Cristina Ortega; Helena M Solo-Gabriele; Gary Miller; Samir Elmir; Xihui Newman; Peter Shih; J Alfredo Bonilla; Tonya D Bonilla; Carol J Palmer; Troy Scott; Jerzy Lukasik; Valerie J Harwood; Shannon McQuaig; Chris Sinigalliano; Maribeth Gidley; Lisa R W Plano; Xiaofang Zhu; John D Wang; Lora E Fleming
Journal:  Appl Environ Microbiol       Date:  2009-12-04       Impact factor: 4.792

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