| Literature DB >> 15186351 |
Lotte Lambertsen1, Claus Sternberg, Søren Molin.
Abstract
The mini-Tn7 transposon system is a convenient tool for site-specific tagging of bacteria in which the tagging DNA is inserted at a unique and neutral chromosomal site. We have expanded the panel of mini-Tn7 delivery plasmids expressing different fluorescent proteins (stable and unstable) from the Escherichia coli lac derived promoter, P(A1/04/03), or from the growth-rate-dependent Escherichia coli promoter PrrnB P1. The mini-Tn7 transposons were inserted and tested in the soil bacterium, Pseudomonas putida KT2440. Successful and site-specific tagging was verified by Southern blots as well as by PCR. Furthermore, the effect of fluorescent protein expression on the cellular growth rate was tested by growth competition assays.Entities:
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Year: 2004 PMID： 15186351 DOI： 10.1111/j.1462-2920.2004.00605.x
Source DB: PubMed Journal: Environ Microbiol ISSN： 1462-2912 Impact factor: 5.491