PURPOSE: To determine the viability of long-term, stored serum, which is used in human in vitro production as a source of energy substrates, amino acids, vitamins, growth factors, and other nutrients. METHOD: Two-cell mouse embryos were used in this prospective, cohort study. Serum stored for 1-, 5-, 10-, and 12-year intervals was subjected to four replications of mouse-embryo testing. RESULT(S): There were no significant differences in blastocyst rates between any of the replicates by year (80-100% blastocyst rate; P > 00.1) or between pooled replicate means by time period. CONCLUSION(S): Serum may be frozen up to 12 years.
PURPOSE: To determine the viability of long-term, stored serum, which is used in human in vitro production as a source of energy substrates, amino acids, vitamins, growth factors, and other nutrients. METHOD: Two-cell mouse embryos were used in this prospective, cohort study. Serum stored for 1-, 5-, 10-, and 12-year intervals was subjected to four replications of mouse-embryo testing. RESULT(S): There were no significant differences in blastocyst rates between any of the replicates by year (80-100% blastocyst rate; P > 00.1) or between pooled replicate means by time period. CONCLUSION(S): Serum may be frozen up to 12 years.
Authors: K E Tucker; B S Hurst; S Guadagnoli; C Dymecki; B Mendelsberg; C A Awoniyi; W D Schlaff Journal: J Assist Reprod Genet Date: 1996-01 Impact factor: 3.412