Literature DB >> 15185365

The molecular mechanism of membrane proteins probed by evanescent infrared waves.

Rebecca M Nyquist1, Kenichi Ataka, Joachim Heberle.   

Abstract

The catalytic action of membrane proteins is vital to many cellular processes. Yet the molecular mechanisms remain poorly understood. We describe here the technique of evanescent infrared difference spectroscopy as a tool to decipher the structural changes associated with the enzymatic action of membrane proteins. Functional changes as minute as the protonation state of individual amino acid side chains can be observed and linked to interactions with a ligand, agonist, effector, or redox partner.

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Year:  2004        PMID: 15185365     DOI: 10.1002/cbic.200300687

Source DB:  PubMed          Journal:  Chembiochem        ISSN: 1439-4227            Impact factor:   3.164


  12 in total

1.  SEIRA spectroscopy on a membrane receptor monolayer using lipoprotein particles as carriers.

Authors:  Ekaterina Zaitseva; Marcia Saavedra; Sourabh Banerjee; Thomas P Sakmar; Reiner Vogel
Journal:  Biophys J       Date:  2010-10-06       Impact factor: 4.033

2.  Stepwise isotope editing of [FeFe]-hydrogenases exposes cofactor dynamics.

Authors:  Moritz Senger; Stefan Mebs; Jifu Duan; Florian Wittkamp; Ulf-Peter Apfel; Joachim Heberle; Michael Haumann; Sven Timo Stripp
Journal:  Proc Natl Acad Sci U S A       Date:  2016-07-18       Impact factor: 11.205

3.  Resolving voltage-dependent structural changes of a membrane photoreceptor by surface-enhanced IR difference spectroscopy.

Authors:  X Jiang; E Zaitseva; M Schmidt; F Siebert; M Engelhard; R Schlesinger; K Ataka; R Vogel; J Heberle
Journal:  Proc Natl Acad Sci U S A       Date:  2008-08-21       Impact factor: 11.205

4.  Redox-linked conformational control of proton-coupled electron transfer: Y122 in the ribonucleotide reductase β2 subunit.

Authors:  Adam R Offenbacher; Lori A Burns; C David Sherrill; Bridgette A Barry
Journal:  J Phys Chem B       Date:  2013-07-03       Impact factor: 2.991

5.  Refolding of a membrane protein in a microfluidics reactor.

Authors:  Nathan R Zaccai; Kamran Yunus; S M Matthews; Adrian C Fisher; Robert J Falconer
Journal:  Eur Biophys J       Date:  2007-01-17       Impact factor: 2.095

6.  Development of a rapid Buffer-exchange system for time-resolved ATR-FTIR spectroscopy with the step-scan mode.

Authors:  Yuji Furutani; Tetsunari Kimura; Kido Okamoto
Journal:  Biophysics (Nagoya-shi)       Date:  2013-08-10

7.  Detection of a protein-bound water vibration of halorhodopsin in aqueous solution.

Authors:  Tetsuya Fukuda; Kosuke Muroda; Hideki Kandori
Journal:  Biophysics (Nagoya-shi)       Date:  2013-12-21

8.  Orientation of non-spherical protonated water clusters revealed by infrared absorption dichroism.

Authors:  Jan O Daldrop; Mattia Saita; Matthias Heyden; Victor A Lorenz-Fonfria; Joachim Heberle; Roland R Netz
Journal:  Nat Commun       Date:  2018-01-22       Impact factor: 14.919

9.  Real-time monitoring of PARP1-dependent PARylation by ATR-FTIR spectroscopy.

Authors:  Annika Krüger; Alexander Bürkle; Karin Hauser; Aswin Mangerich
Journal:  Nat Commun       Date:  2020-05-01       Impact factor: 14.919

10.  Proton transfer and protein conformation dynamics in photosensitive proteins by time-resolved step-scan Fourier-transform infrared spectroscopy.

Authors:  Víctor A Lórenz-Fonfría; Joachim Heberle
Journal:  J Vis Exp       Date:  2014-06-27       Impact factor: 1.355

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