Literature DB >> 15184482

Multiplex PCR assay for rapid detection and genotyping of Helicobacter pylori directly from biopsy specimens.

Santanu Chattopadhyay1, Rajashree Patra, T Ramamurthy, Abhijit Chowdhury, Amal Santra, G K Dhali, S K Bhattacharya, Douglas E Berg, G Balakrish Nair, Asish K Mukhopadhyay.   

Abstract

We developed and evaluated a simple, novel multiplex PCR assay for rapid detection of Helicobacter pylori infection and for the determination of vacA and cagA genotypes directly from gastric biopsy specimens. This assay did not require culturing of strains or extraction of DNA from biopsy samples. This multiplex PCR assay would be of particularly great value for laboratories in developing countries.

Entities:  

Mesh：

Substances：

Year:  2004        PMID： 15184482      PMCID： PMC427847          DOI： 10.1128/JCM.42.6.2821-2824.2004

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  13 in total

1.  Influence of age on 13C-urea breath test results in children.

Authors:  A Kindermann; H Demmelmair; B Koletzko; S Krauss-Etschmann; B Wiebecke; S Koletzko
Journal:  J Pediatr Gastroenterol Nutr       Date:  2000-01       Impact factor: 2.839

2.  Distinctiveness of genotypes of Helicobacter pylori in Calcutta, India.

Authors:  A K Mukhopadhyay; D Kersulyte; J Y Jeong; S Datta; Y Ito; A Chowdhury; S Chowdhury; A Santra; S K Bhattacharya; T Azuma; G B Nair; D E Berg
Journal:  J Bacteriol       Date:  2000-06       Impact factor: 3.490

3.  Simple and accurate PCR-based system for typing vacuolating cytotoxin alleles of Helicobacter pylori.

Authors:  J C Atherton; T L Cover; R J Twells; M R Morales; C J Hawkey; M J Blaser
Journal:  J Clin Microbiol       Date:  1999-09       Impact factor: 5.948

4.  Evaluation of [13C]urea breath test and Helicobacter pylori stool antigen test for diagnosis of H. pylori infection in children from a developing country.

Authors:  Luciana de Carvalho Costa Cardinali; Gifone Aguiar Rocha; Andreia Maria Camargos Rocha; Sílvia Beleza de Moura; Taciana de Figueiredo Soares; Ana Maria Braz Esteves; Ana Margarida Miguel Ferreira Nogueira; Mônica Maria Demas Alvares Cabral; Anfrisina Sales Teles de Carvalho; Paulo Bitencourt; Alexandre Ferreira; Dulciene Maria Magalhães Queiroz
Journal:  J Clin Microbiol       Date:  2003-07       Impact factor: 5.948

5.  Comparison of genotyping Helicobacter pylori directly from biopsy specimens and genotyping from bacterial cultures.

Authors:  Chang-Young Park; Minjung Kwak; Oscar Gutierrez; David Y Graham; Yoshio Yamaoka
Journal:  J Clin Microbiol       Date:  2003-07       Impact factor: 5.948

6.  Helicobacter pylori tissue tropism: mouse-colonizing strains can target different gastric niches.

Authors:  Junko K Akada; Keiji Ogura; Daiva Dailidiene; Giedrius Dailide; James M Cheverud; Douglas E Berg
Journal:  Microbiology       Date:  2003-07       Impact factor: 2.777

7.  Polymerase chain reaction for detection of the cholera enterotoxin operon of Vibrio cholerae.

Authors:  H Shirai; M Nishibuchi; T Ramamurthy; S K Bhattacharya; S C Pal; Y Takeda
Journal:  J Clin Microbiol       Date:  1991-11       Impact factor: 5.948

Review 8.  Unravelling the pathogenic role of Helicobacter pylori in peptic ulcer: potential new therapies and vaccines.

Authors:  J L Telford; A Covacci; P Ghiara; C Montecucco; R Rappuoli
Journal:  Trends Biotechnol       Date:  1994-10       Impact factor: 19.536

9.  Virulence genes in Helicobacter pylori strains from West Bengal residents with overt H. pylori-associated disease and healthy volunteers.

Authors:  Santanu Chattopadhyay; Simanti Datta; Abhijit Chowdhury; Sujit Chowdhury; Asish K Mukhopadhyay; K Rajendran; S K Bhattacharya; Douglas E Berg; G Balakrish Nair
Journal:  J Clin Microbiol       Date:  2002-07       Impact factor: 5.948

10.  Molecular typing of Helicobacter pylori by virulence-gene based multiplex PCR and RT-PCR analysis.

Authors:  Hans-Jürg Monstein; Katarina Ellnebo-Svedlund
Journal:  Helicobacter       Date:  2002-10       Impact factor: 5.753
View more
  34 in total

1.  Prevalence and distribution of Helicobacter pylori cagA and vacA genotypes in the Moroccan population with gastric disease.

Authors:  S Alaoui Boukhris; S A Boukhris; D-a Benajah; K El Rhazi; S A Ibrahimi; C Nejjari; A Amarti; M Mahmoud; M El Abkari; A Souleimani; B Bennani
Journal:  Eur J Clin Microbiol Infect Dis       Date:  2011-12-11       Impact factor: 3.267

2.  In vivo sequence variation in HopZ, a phase-variable outer membrane protein of Helicobacter pylori.

Authors:  Lynn Kennemann; Birgit Brenneke; Sönke Andres; Lars Engstrand; Thomas F Meyer; Toni Aebischer; Christine Josenhans; Sebastian Suerbaum
Journal:  Infect Immun       Date:  2012-10-01       Impact factor: 3.441

3.  Infection with specific Helicobacter pylori-cag pathogenicity island strains is associated with interleukin-1B gene polymorphisms in Venezuelan chronic gastritis patients.

Authors:  Miguel Angel Chiurillo; Yeinmy H Moran; Miryan Cañas; Elvis J Valderrama; Emma Armanie
Journal:  Dig Dis Sci       Date:  2010-06-29       Impact factor: 3.199

4.  Intact cag pathogenicity island of Helicobacter pylori without disease association in Kolkata, India.

Authors:  Rajashree Patra; Santanu Chattopadhyay; Ronita De; Simanti Datta; Abhijit Chowdhury; T Ramamurthy; G Balakrish Nair; Douglas E Berg; Asish K Mukhopadhyay
Journal:  Int J Med Microbiol       Date:  2010-12-30       Impact factor: 3.473

5.  Differential effects of multiplicity of infection on Helicobacter pylori-induced signaling pathways and interleukin-8 gene transcription.

Authors:  Birgit Ritter; Petra Kilian; Marc Rene Reboll; Klaus Resch; Johanna Kay DiStefano; Ronald Frank; Winfried Beil; Mahtab Nourbakhsh
Journal:  J Clin Immunol       Date:  2010-10-01       Impact factor: 8.317

6.  A Mouse Model of Helicobacter pylori Infection.

Authors:  Tanmoy Kumar Dey; Bipul Chandra Karmakar; Avijit Sarkar; Sangita Paul; Asish Kumar Mukhopadhyay
Journal:  Methods Mol Biol       Date:  2021

7.  Susceptibility to Helicobacter pylori infection: results of an epidemiological investigation among gastric cancer patients.

Authors:  Nikola Panic; Elena Mastrostefano; Emanuele Leoncini; Roberto Persiani; Dario Arzani; Rosarita Amore; Riccardo Ricci; Federico Sicoli; Stefano Sioletic; Milutin Bulajic; Domenico D' Ugo; Walter Ricciardi; Stefania Boccia
Journal:  Mol Biol Rep       Date:  2014-02-14       Impact factor: 2.316

8.  Genetic affinities of Helicobacter pylori isolates from ethnic Arabs in Kuwait.

Authors:  M John Albert; Hanan M Al-Akbal; Rita Dhar; Ronita De; Asish K Mukhopadhyay
Journal:  Gut Pathog       Date:  2010-07-05       Impact factor: 4.181

9.  Indistinguishable cellular changes in gastric mucosa between Helicobacter pylori infected asymptomatic tribal and duodenal ulcer patients.

Authors:  Dhira Rani Saha; Simanti Datta; Santanu Chattopadhyay; Rajashree Patra; Ronita De; Krishnan Rajendran; Abhijit Chowdhury; Thandavaryan Ramamurthy; Asish Kumar Mukhopadhyay
Journal:  World J Gastroenterol       Date:  2009-03-07       Impact factor: 5.742

10.  Antimicrobial activity of curcumin against Helicobacter pylori isolates from India and during infections in mice.

Authors:  Ronita De; Parag Kundu; Snehasikta Swarnakar; T Ramamurthy; Abhijit Chowdhury; G Balakrish Nair; Asish K Mukhopadhyay
Journal:  Antimicrob Agents Chemother       Date:  2009-02-09       Impact factor: 5.191
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.