Literature DB >> 15184188

Immobilization of Saccharomyces cerevisiae cystathionine gamma-lyase and application of the product to cystathionine synthesis.

Shuzo Yamagata1, Tsuyoshi Akamatsu, Tomonori Iwama.   

Abstract

Cystathionine gamma-lyase of Saccharomyces cerevisiae was immobilized to aminohexyl-Sepharose through the cofactor pyridoxal 5'-phosphate and was characterized with respect to its cystathionine gamma-synthase activity. The immobilized product was so stable that it repeatedly catalyzed as many as five cycles of the reaction without losing activity.

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Year:  2004        PMID： 15184188      PMCID： PMC427735          DOI： 10.1128/AEM.70.6.3766-3768.2004

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  9 in total

1.  A spectrophotometric method for the direct determination of cysteine in the presence of other naturally occurring amino acids.

Authors:  M K Gaitonde
Journal:  Biochem J       Date:  1967-08       Impact factor: 3.857

2.  A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

Authors:  M M Bradford
Journal:  Anal Biochem       Date:  1976-05-07       Impact factor: 3.365

3.  Preparation of pyridoxal 5'-phosphate-bound sepharose and its use for immobilization of tryptophanase.

Authors:  S Ikeda; S Fukui
Journal:  Biochem Biophys Res Commun       Date:  1973-05-15       Impact factor: 3.575

4.  Protein purification by affinity chromatography. Derivatizations of agarose and polyacrylamide beads.

Authors:  P Cuatrecasas
Journal:  J Biol Chem       Date:  1970-06       Impact factor: 5.157

5.  Comparative studies on the properties of tryptophanase and tyrosine phenol-lyase immobilized directly on Sepharose or by use of Sepharose-bound pyridoxal 5'-phosphate.

Authors:  S Fukui; S Ikeda; M Fujimura; H Yamada; H Kumagai
Journal:  Eur J Biochem       Date:  1975-02-03

6.  Cystathionine gamma-synthase from Arabidopsis thaliana: purification and biochemical characterization of the recombinant enzyme overexpressed in Escherichia coli.

Authors:  S Ravanel; B Gakière; D Job; R Douce
Journal:  Biochem J       Date:  1998-04-15       Impact factor: 3.857

7.  Cysteine biosynthesis in Saccharomyces cerevisiae occurs through the transsulfuration pathway which has been built up by enzyme recruitment.

Authors:  H Cherest; D Thomas; Y Surdin-Kerjan
Journal:  J Bacteriol       Date:  1993-09       Impact factor: 3.490

8.  Cloning and bacterial expression of the CYS3 gene encoding cystathionine gamma-lyase of Saccharomyces cerevisiae and the physicochemical and enzymatic properties of the protein.

Authors:  S Yamagata; R J D'Andrea; S Fujisaki; M Isaji; K Nakamura
Journal:  J Bacteriol       Date:  1993-08       Impact factor: 3.490

9.  Purification and characterization of cystathionine gamma-synthase type II from Bacillus sphaericus.

Authors:  H Kanzaki; M Kobayashi; T Nagasawa; H Yamada
Journal:  Eur J Biochem       Date:  1987-02-16
  9 in total

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