Literature DB >> 15184183

Noninvasive pigment identification in single cells from living phototrophic biofilms by confocal imaging spectrofluorometry.

M Roldán1, F Thomas, S Castel, A Quesada, M Hernández-Mariné.   

Abstract

A new imaging technique for the analysis of fluorescent pigments from a single cell is reported. It is based on confocal scanning laser microscopy coupled with spectrofluorometric methods. The setup allows simultaneous establishment of the relationships among pigment analysis in vivo, morphology, and three-dimensional localization inside thick intact microbial assemblages.

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Year:  2004        PMID: 15184183      PMCID: PMC427743          DOI: 10.1128/AEM.70.6.3745-3750.2004

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN: 0099-2240            Impact factor:   4.792


  11 in total

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