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Literature DB >> 15183179

Large dense-core vesicle exocytosis in pancreatic beta-cells monitored by capacitance measurements.

Takahiro Kanno¹, Xiasong Ma, Sebastian Barg, Lena Eliasson, Juris Galvanovskis, Sven Göpel, Max Larsson, Erik Renström, Patrik Rorsman.

Abstract

This article discusses the currently used methodologies for monitoring exocytosis as changes in cell capacitance. Details are given on composition of solutions, experimental protocols, and how the observed responses can be interpreted physiologically. The concepts are illustrated by examples from our own work on insulin-releasing pancreatic beta-cells. Finally, we consider the feasibility of applying capacitance measurements to endocrine cells in intact pancreatic islets, where the cells are electrically coupled to each other.

Entities: Gene

Mesh：

Year: 2004 PMID： 15183179 DOI： 10.1016/j.ymeth.2004.01.003

Source DB: PubMed Journal: Methods ISSN： 1046-2023 Impact factor: 3.608

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Cited

22 in total

1. Imaging dynamic insulin release using a fluorescent zinc indicator for monitoring induced exocytotic release (ZIMIR).

Authors: Daliang Li; Shiuhwei Chen; Elisa A Bellomo; Andrei I Tarasov; Callan Kaut; Guy A Rutter; Wen-hong Li
Journal: Proc Natl Acad Sci U S A Date: 2011-12-12 Impact factor: 11.205

2. Capacitance measurements of exocytosis in mouse pancreatic alpha-, beta- and delta-cells within intact islets of Langerhans.

Authors: Sven Göpel; Quan Zhang; Lena Eliasson; Xiao-Song Ma; Juris Galvanovskis; Takahiro Kanno; Albert Salehi; Patrik Rorsman
Journal: J Physiol Date: 2004-02-13 Impact factor: 5.182

3. The voltage-dependent potassium channel subunit Kv2.1 regulates insulin secretion from rodent and human islets independently of its electrical function.

Authors: X Q Dai; J E Manning Fox; D Chikvashvili; M Casimir; G Plummer; C Hajmrle; A F Spigelman; T Kin; D Singer-Lahat; Y Kang; A M J Shapiro; H Y Gaisano; I Lotan; P E Macdonald
Journal: Diabetologia Date: 2012-03-13 Impact factor: 10.122

4. Kinetics of Rab27a-dependent actions on vesicle docking and priming in pancreatic beta-cells.

Authors: Matthew J Merrins; Edward L Stuenkel
Journal: J Physiol Date: 2008-09-18 Impact factor: 5.182

5. Ca(2+) clearance by plasmalemmal NCLX, Li(+)-permeable Na(+)/Ca(2+) exchanger, is required for the sustained exocytosis in rat insulinoma INS-1 cells.

Authors: Young-Eun Han; Shin-Young Ryu; Sun-Hyun Park; Kyu-Hee Lee; Suk-Ho Lee; Won-Kyung Ho
Journal: Pflugers Arch Date: 2015-06-24 Impact factor: 3.657

Review 6. Coupling of metabolic, second messenger pathways and insulin granule dynamics in pancreatic beta-cells: a computational analysis.

Authors: Leonid E Fridlyand; Louis H Philipson
Journal: Prog Biophys Mol Biol Date: 2011-09-08 Impact factor: 3.667

Large dense-core vesicle exocytosis in pancreatic beta-cells monitored by capacitance measurements.

1. Imaging dynamic insulin release using a fluorescent zinc indicator for monitoring induced exocytotic release (ZIMIR).

2. Capacitance measurements of exocytosis in mouse pancreatic alpha-, beta- and delta-cells within intact islets of Langerhans.

3. The voltage-dependent potassium channel subunit Kv2.1 regulates insulin secretion from rodent and human islets independently of its electrical function.

4. Kinetics of Rab27a-dependent actions on vesicle docking and priming in pancreatic beta-cells.

5. Ca(2+) clearance by plasmalemmal NCLX, Li(+)-permeable Na(+)/Ca(2+) exchanger, is required for the sustained exocytosis in rat insulinoma INS-1 cells.

Review 6. Coupling of metabolic, second messenger pathways and insulin granule dynamics in pancreatic beta-cells: a computational analysis.

7. Adiponectin induces insulin secretion in vitro and in vivo at a low glucose concentration.

8. Adenylosuccinate Is an Insulin Secretagogue Derived from Glucose-Induced Purine Metabolism.

9. Insulin granule recruitment and exocytosis is dependent on p110gamma in insulinoma and human beta-cells.

10. TCF7L2 regulates late events in insulin secretion from pancreatic islet beta-cells.